Hotspot

trnE-UUC-trnT-GGU

LSC · 38005-39625 · 1621 bp

Back to Cyanotis

Pi 0.185

MarkerSeek score 87.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1621
variable_sites: 260
pi: 0.185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	1559-1690 bp	1.0	89.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	1537-1668 bp	1.0	88.9
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	ACGACTTACGCCTTACCATG	50.0	50.0	57.8	57.7	1588-1719 bp	1.0	88.3
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	ACGACTTACGCCTTACCATG	50.0	50.0	57.7	57.7	1566-1697 bp	1.0	87.4
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	CGACTTACGCCTTACCATGG	50.0	55.0	57.8	58.4	1587-1718 bp	1.0	87.2