Hotspot

petN-psbM

LSC · 33155-36117 · 2963 bp

Back to Cyanotis

Pi 0.0665

MarkerSeek score 63.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 2963
variable_sites: 98
pi: 0.0665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.50
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AAAAATTGCAGGGGTCGACT	ACGGATTCAAACTCCAAGGT	45.0	45.0	58.0	57.0	718-2163 bp	1.0	79.5
petN-psbM_p2	GTTCCCTTGAGAAATCCGCA	ACGGATTCAAACTCCAAGGT	50.0	45.0	58.2	57.0	817-2262 bp	1.0	77.9
petN-psbM_p3	TGTTCCCTTGAGAAATCCGC	ACGGATTCAAACTCCAAGGT	50.0	45.0	58.2	57.0	818-2263 bp	1.0	77.9
petN-psbM_p4	ATTGCAGGGGTCGACTCTAT	ACGGATTCAAACTCCAAGGT	50.0	45.0	58.2	57.0	714-2159 bp	1.0	77.9
petN-psbM_p5	AAAAATTGCAGGGGTCGACT	ACGGATTCAAACTCCAAGGTT	45.0	42.9	58.0	57.8	718-2163 bp	1.0	77.3