Hotspot

rpoB-trnC-GCA

LSC · 30604-32009 · 1406 bp

Back to Cyanotis

Pi 0.0647

MarkerSeek score 80.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1406
variable_sites: 89
pi: 0.0647
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTGGACATTCCCTCATTCCC	TTACCACTTGGCTATGTCGC	55.0	50.0	57.9	58.0	1462-1643 bp	1.0	85.5
rpoB-trnC-GCA_p2	CCCTCATTCCCATCATTCCG	TTACCACTTGGCTATGTCGC	55.0	50.0	58.1	58.0	1453-1634 bp	1.0	85.2
rpoB-trnC-GCA_p3	CCTCATTCCCATCATTCCGG	TTACCACTTGGCTATGTCGC	55.0	50.0	58.1	58.0	1452-1633 bp	1.0	85.2
rpoB-trnC-GCA_p4	GTGGACATTCCCTCATTCCC	AAAGGATTTGCAGTCCCCTG	55.0	50.0	57.9	58.1	1484-1665 bp	1.0	85.0
rpoB-trnC-GCA_p5	CCTCATTCCCATCATTCCGG	AAAGGATTTGCAGTCCCCTG	55.0	50.0	58.1	58.1	1474-1655 bp	1.0	84.7