Hotspot

rps2-rpoC2

LSC · 19908-20155 · 248 bp

Back to Cyanotis

Pi 0.12

MarkerSeek score 78.20

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 248
variable_sites: 29
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	TGTATGGGGCCATTTTTGGA	TGAAATGAATTTACATGAGACAT	45.0	26.1	57.4	52.4	376 bp	1.0	47.7
rps2-rpoC2_p2	TGTATGGGGCCATTTTTGGA	ATGAAATGAATTTACATGAGACA	45.0	26.1	57.4	52.4	377 bp	1.0	47.6
rps2-rpoC2_p3	GGAGATGTATGGGGCCATTT	TGAAATGAATTTACATGAGACAT	50.0	26.1	57.3	52.4	381 bp	1.0	47.6
rps2-rpoC2_p4	CTTTGCGGAGATGTATGGGG	ATGAAATGAATTTACATGAGACA	55.0	26.1	58.4	52.4	388 bp	1.0	47.4
rps2-rpoC2_p5	CTTTGCGGAGATGTATGGGG	TGAAATGAATTTACATGAGACAT	55.0	26.1	58.4	52.4	387 bp	1.0	47.4