Hotspot

trnS-GCU-trnR-UCU

LSC · 9912-13093 · 3182 bp

Back to Cyanotis

Pi 0.114
MarkerSeek score 71.70
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
3182
variable_sites
209
pi
0.114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.58
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 ATTAGCAATCCGCCGCTTTA AGCATTAAACTGGTATTAGAAGCA 45.0 33.3 58.0 56.4 1328 bp 0.5 20.0
trnS-GCU-trnR-UCU_p2 ACTCAGCCATCTCTCCCAAT AGCATTAAACTGGTATTAGAAGCA 50.0 33.3 58.1 56.4 1304 bp 0.5 20.0
trnS-GCU-trnR-UCU_p3 ATTAGCAATCCGCCGCTTTA AAGCATTAAACTGGTATTAGAAGCA 45.0 32.0 58.0 57.1 1329 bp 0.5 20.0
trnS-GCU-trnR-UCU_p4 GCTTTAGTCCACTCAGCCAT AGCATTAAACTGGTATTAGAAGCA 50.0 33.3 57.6 56.4 1314 bp 0.5 20.0
trnS-GCU-trnR-UCU_p5 ATTAGCAATCCGCCGCTTTA GCATTAAACTGGTATTAGAAGCA 45.0 34.8 58.0 55.0 1327 bp 0.5 20.0