Hotspot

mntB

SSC · 26814-27698 · 885 bp

Back to Cyanophora

Pi 0.426

MarkerSeek score 81.30

Primer available yes

Label mntB

Feature Metadata

label_name: mntB
feature_type: gene
region: SSC
length_bp: 885
variable_sites: 327
pi: 0.426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
mntB_p1	TGGGTGCCGATTTGATTGAA	CGTGAACTTTCTGGTGGACA	45.0	50.0	58.1	58.1	1842 bp	0.5	58.9
mntB_p2	TGGGTGCCGATTTGATTGAA	GTTATGTTCCACAACGTGCG	45.0	50.0	58.1	58.1	2021 bp	0.5	58.9
mntB_p3	TGGGTGCCGATTTGATTGAA	GGACCAAATGGAGCAGGAAA	45.0	50.0	58.1	58.1	2139 bp	0.5	58.7
mntB_p4	TGGGTGCCGATTTGATTGAA	GCTCGTTTACTTGCTCAGGA	45.0	50.0	58.1	57.9	1803 bp	0.5	58.7
mntB_p5	TGGGTGCCGATTTGATTGAA	AGCTCGTTTACTTGCTCAGG	45.0	50.0	58.1	57.9	1804 bp	0.5	58.6