Hotspot

crtE

SSC · 32445-33356 · 912 bp

Back to Cyanophora

Pi 0.435

MarkerSeek score 83.30

Primer available yes

Label crtE

Feature Metadata

label_name: crtE
feature_type: gene
region: SSC
length_bp: 912
variable_sites: 378
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
crtE_p1	TTTGGCCCTCAGTATGCATC	GCAAGCAGCTCGCATTTTTA	50.0	45.0	57.9	58.0	1422 bp	0.5	59.4
crtE_p2	TTTGGCCCTCAGTATGCATC	TGTGCAATCGGGATTAGGTG	50.0	50.0	57.9	58.0	1569 bp	0.5	59.2
crtE_p3	TGGCCCTCAGTATGCATCTA	GCAAGCAGCTCGCATTTTTA	50.0	45.0	57.9	58.0	1420 bp	0.5	59.0
crtE_p4	TTCGATCTTTCCCCAAGCTG	GCAAGCAGCTCGCATTTTTA	50.0	45.0	57.9	58.0	1266 bp	0.5	58.9
crtE_p5	GTTCGATCTTTCCCCAAGCT	GCAAGCAGCTCGCATTTTTA	50.0	45.0	57.9	58.0	1267 bp	0.5	58.9