Hotspot

cpcB

SSC · 31240-31758 · 519 bp

Back to Cyanophora

Pi 0.406

MarkerSeek score 81.50

Primer available yes

Label cpcB

Feature Metadata

label_name: cpcB
feature_type: gene
region: SSC
length_bp: 519
variable_sites: 201
pi: 0.406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cpcB_p1	GTGGTCCAGCATCCCATTTA	GATGCATACTGAGGGCCAAA	50.0	50.0	57.6	57.9	1326 bp	0.5	56.1
cpcB_p2	GTGGTCCAGCATCCCATTTA	TAGATGCATACTGAGGGCCA	50.0	50.0	57.6	57.9	1328 bp	0.5	55.7
cpcB_p3	GTGGTCCAGCATCCCATTTA	CAGCTTGGGGAAAGATCGAA	50.0	50.0	57.6	57.9	1482 bp	0.5	55.6
cpcB_p4	GTGGTCCAGCATCCCATTTA	AGCTTGGGGAAAGATCGAAC	50.0	50.0	57.6	57.9	1481 bp	0.5	55.6
cpcB_p5	GTGGTCCAGCATCCCATTTA	ACGCACGGTTCATTTCTTCT	50.0	45.0	57.6	58.1	1463 bp	0.5	55.5