Hotspot

apcF

SSC · 29460-29969 · 510 bp

Back to Cyanophora

Pi 0.403

MarkerSeek score 81.10

Primer available yes

Label apcF

Feature Metadata

label_name: apcF
feature_type: gene
region: SSC
length_bp: 510
variable_sites: 189
pi: 0.403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
apcF_p1	CCAAAGTGGACTACGAATAAACC	ACTCCTACAGGAAGCCGAAT	43.5	50.0	57.5	58.1	972 bp	0.5	30.6
apcF_p2	CCAAAGTGGACTACGAATAAACC	CAAACTCCTACAGGAAGCCG	43.5	55.0	57.5	58.3	975 bp	0.5	29.5
apcF_p3	CCAAAGTGGACTACGAATAAACC	AAACTCCTACAGGAAGCCGA	43.5	50.0	57.5	58.4	974 bp	0.5	28.8
apcF_p4	CCAAAGTGGACTACGAATAAACC	AACTCCTACAGGAAGCCGAA	43.5	50.0	57.5	58.4	973 bp	0.5	28.8
apcF_p5	TCGGTTCAATTTCAAATTGGAGT	ACTCCTACAGGAAGCCGAAT	34.8	50.0	57.2	58.1	1009 bp	0.5	28.7