Hotspot

trnM-trnF

LSC · 122424-122579 · 156 bp

Back to Cyanophora

Pi 0.247

MarkerSeek score 81.70

Primer available yes

Label trnM-trnF

Feature Metadata

label_name: trnM-trnF
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 37
pi: 0.247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-trnF_p1	CGGGCTCATAATCCGTCATC	TCCACTGCTCTACCAACTGA	55.0	50.0	58.3	58.0	245-266 bp	1.0	87.5
trnM-trnF_p2	CGGGCTCATAATCCGTCATC	GCGGATTTTCAATCCACTGC	55.0	50.0	58.3	58.1	257-278 bp	1.0	86.8
trnM-trnF_p3	CGGGCTCATAATCCGTCATC	CACGCGGATTTTCAATCCAC	55.0	50.0	58.3	58.1	260-281 bp	1.0	86.7
trnM-trnF_p4	CGGGCTCATAATCCGTCATC	AGACTTGAACTCGTGACACG	55.0	50.0	58.3	57.9	276-297 bp	1.0	86.4
trnM-trnF_p5	CGGGCTCATAATCCGTCATC	GACACGCGGATTTTCAATCC	55.0	50.0	58.3	57.8	262-283 bp	1.0	86.1