Hotspot

ORF310

LSC · 33324-34256 · 933 bp

Pi 0.0751

MarkerSeek score 78.40

Primer available yes

Label ORF310

Feature Metadata

label_name: ORF310
feature_type: gene
region: LSC
length_bp: 933
variable_sites: 68
pi: 0.0751
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF310_p1	CCTGATCGATGAACCTGCAA	GCCGCCAAAATCATCCAAAA	50.0	45.0	58.0	57.9	1882-2037 bp	1.0	87.9
ORF310_p2	AGTCCCGCGGATACATAGAA	GCCGCCAAAATCATCCAAAA	50.0	45.0	58.0	57.9	2051-2206 bp	1.0	87.8
ORF310_p3	AGTCCCGCGGATACATAGAA	GCACCTATTACCGCACTTGA	50.0	50.0	58.0	58.0	1857 bp	0.5	59.8
ORF310_p4	CCTGATCGATGAACCTGCAA	GCACCTATTACCGCACTTGA	50.0	50.0	58.0	58.0	1688 bp	0.5	59.6
ORF310_p5	GTATGTGGTAGGGCGAAAGG	GCACCTATTACCGCACTTGA	55.0	50.0	58.1	58.0	1401 bp	0.5	59.4