Hotspot

ORF310-trnC (GCA)

LSC · 34257-34787 · 531 bp

Back to Cyanea

Pi 0.0852
MarkerSeek score 75.90
Primer available yes
Label ORF310-trnC (GCA)

Feature Metadata

label_name
ORF310-trnC (GCA)
feature_type
igs
region
LSC
length_bp
531
variable_sites
38
pi
0.0852
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF310-trnC (GCA)_p1 GACCAAAACCCAACCCTGAT GGTGCAGAGTCAAAAAGGGA 50.0 50.0 58.0 58.0 1205-1284 bp 1.0 88.6
ORF310-trnC (GCA)_p2 ATTTCGTAGACGTCCTTGGC TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.0 58.0 1117 bp 0.5 59.9
ORF310-trnC (GCA)_p3 GACCAAAACCCAACCCTGAT TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.0 58.0 1387 bp 0.5 59.9
ORF310-trnC (GCA)_p4 ATTTCGTAGACGTCCTTGGC GGTGCAGAGTCAAAAAGGGA 50.0 50.0 58.0 58.0 1014 bp 0.5 59.8
ORF310-trnC (GCA)_p5 ATGGCGTCTGGTTTGTTTCT TTAAAGCAGCCCAAGCAAGA 45.0 45.0 58.0 58.0 1543 bp 0.5 59.8