Hotspot

trnQ (UUG)-psbK

LSC · 12848-13462 · 615 bp

Back to Cyanea

Pi 0.0787
MarkerSeek score 79.20
Primer available yes
Label trnQ (UUG)-psbK

Feature Metadata

label_name
trnQ (UUG)-psbK
feature_type
igs
region
LSC
length_bp
615
variable_sites
42
pi
0.0787
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ (UUG)-psbK_p1 CTTACCACTCGGCTACGC ACAGATACAGATCAAACTTAAAAGA 61.1 28.0 57.6 54.5 604-670 bp 1.0 49.3
trnQ (UUG)-psbK_p2 CTTACCACTCGGCTACGC AACAGATACAGATCAAACTTAAAAGA 61.1 26.9 57.6 55.2 605-671 bp 1.0 49.3
trnQ (UUG)-psbK_p3 CTTACCACTCGGCTACGC ACAGATACAGATCAAACTTAAAAG 61.1 29.2 57.6 53.1 604-670 bp 1.0 49.3
trnQ (UUG)-psbK_p4 CGCCTTACCACTCGGCTA ACAGATACAGATCAAACTTAAAAGA 61.1 28.0 58.8 54.5 607-673 bp 1.0 49.3
trnQ (UUG)-psbK_p5 GCCTTACCACTCGGCTAC ACAGATACAGATCAAACTTAAAAGA 61.1 28.0 57.2 54.5 606-672 bp 1.0 49.3