Hotspot

rps16-ORF243

LSC · 11546-11707 · 162 bp

Back to Cyanea

Pi 0.106
MarkerSeek score 76.80
Primer available yes
Label rps16-ORF243

Feature Metadata

label_name
rps16-ORF243
feature_type
igs
region
LSC
length_bp
162
variable_sites
16
pi
0.106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-ORF243_p1 AGCGAACATCAATTGCAACG AAAAAGTACGAACCGGTGCT 45.0 45.0 58.0 58.0 546-547 bp 1.0 89.0
rps16-ORF243_p2 AGCGAACATCAATTGCAACG GCCCTCCAAGCGAATCTAAA 45.0 50.0 58.0 58.0 370 bp 0.5 59.4
rps16-ORF243_p3 AGCGAACATCAATTGCAACG ACGTTGGATTTTGTCACGGA 45.0 45.0 58.0 58.0 297 bp 0.5 59.4
rps16-ORF243_p4 GCGAACATCAATTGCAACGA GCCCTCCAAGCGAATCTAAA 45.0 50.0 58.0 58.0 369 bp 0.5 59.4
rps16-ORF243_p5 TGCTTTCTACCACAGCGTTT GCCCTCCAAGCGAATCTAAA 45.0 50.0 58.0 58.0 332 bp 0.5 59.4