Hotspot

trnM (CAU)-trnV (UAC)

LSC · 6927-7123 · 197 bp

Back to Cyanea

Pi 0.0428
MarkerSeek score 74.50
Primer available yes
Label trnM (CAU)-trnV (UAC)

Feature Metadata

label_name
trnM (CAU)-trnV (UAC)
feature_type
igs
region
LSC
length_bp
197
variable_sites
8
pi
0.0428
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM (CAU)-trnV (UAC)_p1 CCCGCCGTATGAAAGCAATA ACCAACTGAGCTATAGCCCT 50.0 50.0 58.4 57.8 251-261 bp 1.0 80.9
trnM (CAU)-trnV (UAC)_p2 CCCGCCGTATGAAAGCAATA TTGCATGATGGACTCCCTTG 50.0 50.0 58.4 57.9 305-315 bp 1.0 80.3
trnM (CAU)-trnV (UAC)_p3 CCCGCCGTATGAAAGCAATA TGATTGCATGATGGACTCCC 50.0 50.0 58.4 57.6 308-318 bp 1.0 78.5
trnM (CAU)-trnV (UAC)_p4 CCGCCGTATGAAAGCAATAC TTGCATGATGGACTCCCTTG 50.0 50.0 57.3 57.9 304-314 bp 1.0 78.3
trnM (CAU)-trnV (UAC)_p5 CCCGCCGTATGAAAGCAATA GCATGATGGACTCCCTTGAA 50.0 50.0 58.4 57.6 303-313 bp 1.0 78.0