Hotspot

psbA-rbcL

LSC · 1474-2535 · 1062 bp

Back to Cyanea

Pi 0.0291
MarkerSeek score 79.80
Primer available yes
Label psbA-rbcL

Feature Metadata

label_name
psbA-rbcL
feature_type
igs
region
LSC
length_bp
1062
variable_sites
29
pi
0.0291
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-rbcL_p1 TACAGAAGCGACCCCATAGG ATTTGAGTTTGCCGCAATGG 55.0 45.0 58.6 57.9 1098-1148 bp 1.0 80.7
psbA-rbcL_p2 TACAGAAGCGACCCCATAGG GCTGCTTGTGAGGTATGGAA 55.0 50.0 58.6 57.9 1127-1177 bp 1.0 80.4
psbA-rbcL_p3 TACAGAAGCGACCCCATAGG TGAGTTTGCCGCAATGGATA 55.0 45.0 58.6 57.9 1095-1145 bp 1.0 80.4
psbA-rbcL_p4 TACAGAAGCGACCCCATAGG AGTTTGCCGCAATGGATACT 55.0 45.0 58.6 57.9 1093-1143 bp 1.0 80.3
psbA-rbcL_p5 ATCCAGTTACAGAAGCGACC ATTTGAGTTTGCCGCAATGG 50.0 45.0 57.3 57.9 1105-1155 bp 1.0 80.0