primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-rbcL_p1	psbA-rbcL	1	TACAGAAGCGACCCCATAGG	ATTTGAGTTTGCCGCAATGG	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.673162	1474	2535	1098-1148	1.000000	80.7
psbA-rbcL_p2	psbA-rbcL	2	TACAGAAGCGACCCCATAGG	GCTGCTTGTGAGGTATGGAA	20	20	55.0	50.0	58.6	57.9	0.0	0.0	0.703856	1474	2535	1127-1177	1.000000	80.4
psbA-rbcL_p3	psbA-rbcL	3	TACAGAAGCGACCCCATAGG	TGAGTTTGCCGCAATGGATA	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.711287	1474	2535	1095-1145	1.000000	80.4
psbA-rbcL_p4	psbA-rbcL	4	TACAGAAGCGACCCCATAGG	AGTTTGCCGCAATGGATACT	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.718766	1474	2535	1093-1143	1.000000	80.3
psbA-rbcL_p5	psbA-rbcL	5	ATCCAGTTACAGAAGCGACC	ATTTGAGTTTGCCGCAATGG	20	20	50.0	45.0	57.3	57.9	0.0	6.6	0.757962	1474	2535	1105-1155	1.000000	80.0
trnM (CAU)-trnV (UAC)_p1	trnM (CAU)-trnV (UAC)	1	CCCGCCGTATGAAAGCAATA	ACCAACTGAGCTATAGCCCT	20	20	50.0	50.0	58.4	57.8	0.0	21.2	0.593842	6927	7123	251-261	1.000000	80.9
trnM (CAU)-trnV (UAC)_p2	trnM (CAU)-trnV (UAC)	2	CCCGCCGTATGAAAGCAATA	TTGCATGATGGACTCCCTTG	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.544607	6927	7123	305-315	1.000000	80.3
trnM (CAU)-trnV (UAC)_p3	trnM (CAU)-trnV (UAC)	3	CCCGCCGTATGAAAGCAATA	TGATTGCATGATGGACTCCC	20	20	50.0	50.0	58.4	57.6	0.0	0.0	0.764682	6927	7123	308-318	1.000000	78.5
trnM (CAU)-trnV (UAC)_p4	trnM (CAU)-trnV (UAC)	4	CCGCCGTATGAAAGCAATAC	TTGCATGATGGACTCCCTTG	20	20	50.0	50.0	57.3	57.9	0.0	0.0	0.796560	6927	7123	304-314	1.000000	78.3
trnM (CAU)-trnV (UAC)_p5	trnM (CAU)-trnV (UAC)	5	CCCGCCGTATGAAAGCAATA	GCATGATGGACTCCCTTGAA	20	20	50.0	50.0	58.4	57.6	0.0	0.0	0.836469	6927	7123	303-313	1.000000	78.0
rps16-ORF243_p1	rps16-ORF243	1	AGCGAACATCAATTGCAACG	AAAAAGTACGAACCGGTGCT	20	20	45.0	45.0	58.0	58.0	0.0	8.5	0.074570	11546	11707	546-547	1.000000	89.0
rps16-ORF243_p2	rps16-ORF243	2	AGCGAACATCAATTGCAACG	GCCCTCCAAGCGAATCTAAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.070625	11546	11707	370-370	0.500000	59.4
rps16-ORF243_p3	rps16-ORF243	3	AGCGAACATCAATTGCAACG	ACGTTGGATTTTGTCACGGA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.075942	11546	11707	297-297	0.500000	59.4
rps16-ORF243_p4	rps16-ORF243	4	GCGAACATCAATTGCAACGA	GCCCTCCAAGCGAATCTAAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.075975	11546	11707	369-369	0.500000	59.4
rps16-ORF243_p5	rps16-ORF243	5	TGCTTTCTACCACAGCGTTT	GCCCTCCAAGCGAATCTAAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.076593	11546	11707	332-332	0.500000	59.4
ORF243_p1	ORF243	1	AGCGAACATCAATTGCAACG	CAAAGATTGCTGCGGGAAAG	20	20	45.0	50.0	58.0	58.0	0.0	2.7	0.045540	11708	12439	2014-2294	1.000000	91.6
ORF243_p2	ORF243	2	GCGAACATCAATTGCAACGA	CAAAGATTGCTGCGGGAAAG	20	20	45.0	50.0	58.0	58.0	0.0	2.7	0.050890	11708	12439	2013-2293	1.000000	91.6
ORF243_p3	ORF243	3	TGCTTTCTACCACAGCGTTT	CAAAGATTGCTGCGGGAAAG	20	20	45.0	50.0	58.0	58.0	0.0	2.7	0.051509	11708	12439	1976-2256	1.000000	91.6
ORF243_p4	ORF243	4	TTGCTTTCTACCACAGCGTT	CAAAGATTGCTGCGGGAAAG	20	20	45.0	50.0	58.0	58.0	0.0	2.7	0.051509	11708	12439	1977-2257	1.000000	91.6
ORF243_p5	ORF243	5	AGCGAACATCAATTGCAACG	GGCAAATCGTCCCAATTTCG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.124173	11708	12439	1242-1242	0.500000	59.0
ORF243-trnQ (UUG)_p1	ORF243-trnQ (UUG)	1	AGCGAACATCAATTGCAACG	CAAAGATTGCTGCGGGAAAG	20	20	45.0	50.0	58.0	58.0	0.0	2.7	0.045540	12440	12775	2014-2294	1.000000	91.6
ORF243-trnQ (UUG)_p2	ORF243-trnQ (UUG)	2	GCGAACATCAATTGCAACGA	CAAAGATTGCTGCGGGAAAG	20	20	45.0	50.0	58.0	58.0	0.0	2.7	0.050890	12440	12775	2013-2293	1.000000	91.6
ORF243-trnQ (UUG)_p3	ORF243-trnQ (UUG)	3	TGCTTTCTACCACAGCGTTT	CAAAGATTGCTGCGGGAAAG	20	20	45.0	50.0	58.0	58.0	0.0	2.7	0.051509	12440	12775	1976-2256	1.000000	91.6
ORF243-trnQ (UUG)_p4	ORF243-trnQ (UUG)	4	TTGCTTTCTACCACAGCGTT	CAAAGATTGCTGCGGGAAAG	20	20	45.0	50.0	58.0	58.0	0.0	2.7	0.051509	12440	12775	1977-2257	1.000000	91.6
ORF243-trnQ (UUG)_p5	ORF243-trnQ (UUG)	5	TTTAGATTCGCTTGGAGGGC	CAAAGATTGCTGCGGGAAAG	20	20	50.0	50.0	58.0	58.0	0.0	2.7	0.051689	12440	12775	1944-1944	0.500000	59.6
trnQ (UUG)-psbK_p1	trnQ (UUG)-psbK	1	CTTACCACTCGGCTACGC	ACAGATACAGATCAAACTTAAAAGA	18	25	61.1	28.0	57.6	54.5	0.0	0.0	10.919762	12848	13462	604-670	1.000000	49.3
trnQ (UUG)-psbK_p2	trnQ (UUG)-psbK	2	CTTACCACTCGGCTACGC	AACAGATACAGATCAAACTTAAAAGA	18	26	61.1	26.9	57.6	55.2	0.0	0.0	11.228991	12848	13462	605-671	1.000000	49.3
trnQ (UUG)-psbK_p3	trnQ (UUG)-psbK	3	CTTACCACTCGGCTACGC	ACAGATACAGATCAAACTTAAAAG	18	24	61.1	29.2	57.6	53.1	0.0	0.0	11.311587	12848	13462	604-670	1.000000	49.3
trnQ (UUG)-psbK_p4	trnQ (UUG)-psbK	4	CGCCTTACCACTCGGCTA	ACAGATACAGATCAAACTTAAAAGA	18	25	61.1	28.0	58.8	54.5	0.0	0.0	11.319467	12848	13462	607-673	1.000000	49.3
trnQ (UUG)-psbK_p5	trnQ (UUG)-psbK	5	GCCTTACCACTCGGCTAC	ACAGATACAGATCAAACTTAAAAGA	18	25	61.1	28.0	57.2	54.5	0.0	0.0	11.352496	12848	13462	606-672	1.000000	49.3
rpoC2-rpoC1_p1	rpoC2-rpoC1	1	TGTGAAAGACCAGATTGGCC	GCGAAATCGAAGAGGCCATA	20	20	50.0	50.0	58.1	58.1	0.0	5.0	0.224435	26616	26781	253-253	1.000000	80.7
rpoC2-rpoC1_p2	rpoC2-rpoC1	2	TGTGAAAGACCAGATTGGCC	GAGGCCATACAGGGGTTTTC	20	20	50.0	55.0	58.1	58.2	0.0	5.0	0.326332	26616	26781	242-242	1.000000	80.0
rpoC2-rpoC1_p3	rpoC2-rpoC1	3	TGTGAAAGACCAGATTGGCC	TATCGCGAAATCGAAGAGGC	20	20	50.0	50.0	58.1	58.2	0.0	15.2	0.323012	26616	26781	257-257	1.000000	79.9
rpoC2-rpoC1_p4	rpoC2-rpoC1	4	TGTGAAAGACCAGATTGGCC	TTTCCCAGGCCTGTTCCTAT	20	20	50.0	50.0	58.1	58.3	0.0	30.6	0.409072	26616	26781	226-226	1.000000	79.6
rpoC2-rpoC1_p5	rpoC2-rpoC1	5	TGTGAAAGACCAGATTGGCC	TTTTCCCAGGCCTGTTCCTA	20	20	50.0	50.0	58.1	58.5	0.0	30.6	0.638851	26616	26781	227-227	1.000000	77.7
rpoC1-rpoB_p1	rpoC1-rpoB	1	TGTTGCGGAGAAACTAATCCA	TTGGCTCTGGAACTGAATCA	21	20	42.9	45.0	57.6	56.7	0.0	0.0	2.661290	29589	29631	168-168	1.000000	60.1
rpoC1-rpoB_p2	rpoC1-rpoB	2	TGTTGCGGAGAAACTAATCCA	TCTTTGGCTCTGGAACTGAA	21	20	42.9	45.0	57.6	56.7	0.0	0.0	2.733493	29589	29631	171-171	1.000000	59.5
rpoC1-rpoB_p3	rpoC1-rpoB	3	TGTTGCGGAGAAACTAATCCA	TGGCTCTGGAACTGAATCAT	21	20	42.9	45.0	57.6	56.5	0.0	0.0	2.894652	29589	29631	167-167	1.000000	58.3
rpoC1-rpoB_p4	rpoC1-rpoB	4	TGTTGCGGAGAAACTAATCCA	TTTGGCTCTGGAACTGAATCA	21	21	42.9	42.9	57.6	57.5	0.0	0.0	2.917792	29589	29631	169-169	1.000000	58.1
rpoC1-rpoB_p5	rpoC1-rpoB	5	TGTTGCGGAGAAACTAATCCA	TGGCTCTGGAACTGAATCATT	21	21	42.9	42.9	57.6	57.3	0.0	0.0	3.137188	29589	29631	167-167	1.000000	56.3
ORF310_p1	ORF310	1	CCTGATCGATGAACCTGCAA	GCCGCCAAAATCATCCAAAA	20	20	50.0	45.0	58.0	57.9	0.5	0.0	0.120272	33324	34256	1882-2037	1.000000	87.9
ORF310_p2	ORF310	2	AGTCCCGCGGATACATAGAA	GCCGCCAAAATCATCCAAAA	20	20	50.0	45.0	58.0	57.9	30.7	0.0	0.100731	33324	34256	2051-2206	1.000000	87.8
ORF310_p3	ORF310	3	AGTCCCGCGGATACATAGAA	GCACCTATTACCGCACTTGA	20	20	50.0	50.0	58.0	58.0	30.7	0.0	0.027421	33324	34256	1857-1857	0.500000	59.8
ORF310_p4	ORF310	4	CCTGATCGATGAACCTGCAA	GCACCTATTACCGCACTTGA	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.046962	33324	34256	1688-1688	0.500000	59.6
ORF310_p5	ORF310	5	GTATGTGGTAGGGCGAAAGG	GCACCTATTACCGCACTTGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.069077	33324	34256	1401-1401	0.500000	59.4
ORF310-trnC (GCA)_p1	ORF310-trnC (GCA)	1	GACCAAAACCCAACCCTGAT	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028425	34257	34787	1205-1284	1.000000	88.6
ORF310-trnC (GCA)_p2	ORF310-trnC (GCA)	2	ATTTCGTAGACGTCCTTGGC	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.0	58.0	13.0	0.0	0.012015	34257	34787	1117-1117	0.500000	59.9
ORF310-trnC (GCA)_p3	ORF310-trnC (GCA)	3	GACCAAAACCCAACCCTGAT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.015725	34257	34787	1387-1387	0.500000	59.9
ORF310-trnC (GCA)_p4	ORF310-trnC (GCA)	4	ATTTCGTAGACGTCCTTGGC	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	13.0	0.0	0.024714	34257	34787	1014-1014	0.500000	59.8
ORF310-trnC (GCA)_p5	ORF310-trnC (GCA)	5	ATGGCGTCTGGTTTGTTTCT	TTAAAGCAGCCCAAGCAAGA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.024788	34257	34787	1543-1543	0.500000	59.8
ndhC-ORF225_p1	ndhC-ORF225	1	CTAGAAAGTTTCTCCGGCCC	CTCGGGCAAAGTCGAATACA	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.030171	56919	57357	969-1027	1.000000	91.1
ndhC-ORF225_p2	ndhC-ORF225	2	CTAGAAAGTTTCTCCGGCCC	CACTCGGGCAAAGTCGAATA	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.030171	56919	57357	971-1029	1.000000	91.0
ndhC-ORF225_p3	ndhC-ORF225	3	CTAGAAAGTTTCTCCGGCCC	ACTCGGGCAAAGTCGAATAC	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.031559	56919	57357	970-1028	1.000000	91.0
ndhC-ORF225_p4	ndhC-ORF225	4	TCTCACTAAAGAAGGGGCCA	CACTCGGGCAAAGTCGAATA	20	20	50.0	50.0	58.0	58.0	4.4	0.0	0.043445	56919	57357	1529-1587	1.000000	87.5
ndhC-ORF225_p5	ndhC-ORF225	5	TCTCACTAAAGAAGGGGCCA	CTCGGGCAAAGTCGAATACA	20	20	50.0	50.0	58.0	58.0	4.4	0.0	0.043445	56919	57357	1527-1585	1.000000	87.5
ORF225_p1	ORF225	1	CTAGAAAGTTTCTCCGGCCC	TCGACAGAAGCTGCTTCATC	20	20	55.0	50.0	58.0	58.0	21.2	35.8	0.027401	57358	58035	1541-1541	0.500000	59.8
ORF225_p2	ORF225	2	CTAGAAAGTTTCTCCGGCCC	CGACAGAAGCTGCTTCATCT	20	20	55.0	50.0	58.0	58.0	21.2	35.8	0.032949	57358	58035	1540-1540	0.500000	59.7
ORF225_p3	ORF225	3	AAAGCAGAAGCATAGACGCA	TCGACAGAAGCTGCTTCATC	20	20	45.0	50.0	57.9	58.0	0.0	35.8	0.100953	57358	58035	1426-1426	0.500000	59.2
ORF225_p4	ORF225	4	AAACAACAAAGGAGGCCCAT	TCGACAGAAGCTGCTTCATC	20	20	45.0	50.0	57.9	58.0	0.0	35.8	0.103452	57358	58035	997-997	0.500000	59.2
ORF225_p5	ORF225	5	AAAGCAGAAGCATAGACGCA	CGACAGAAGCTGCTTCATCT	20	20	45.0	50.0	57.9	58.0	0.0	35.8	0.106501	57358	58035	1425-1425	0.500000	59.1
rpl16-rps3_p1	rpl16-rps3	1	AAACCAACGAGTCACACACT	CGAACTATTTCTGGGATATTAGGC	20	24	45.0	41.7	57.6	57.0	0.0	0.0	5.366418	81116	81280	248-249	1.000000	43.5
rpl16-rps3_p2	rpl16-rps3	2	AAACCAACGAGTCACACACT	TCGAACTATTTCTGGGATATTAGGC	20	25	45.0	40.0	57.6	58.3	0.0	0.0	5.698210	81116	81280	249-250	1.000000	43.5
rpl16-rps3_p3	rpl16-rps3	3	TGCGAAGTGATGAATTGGGT	CGAACTATTTCTGGGATATTAGGC	20	24	45.0	41.7	57.8	57.0	0.0	0.0	5.168655	81116	81280	323-325	1.000000	42.7
rpl16-rps3_p4	rpl16-rps3	4	TGCGAAGTGATGAATTGGGT	TCGAACTATTTCTGGGATATTAGGC	20	25	45.0	40.0	57.8	58.3	0.0	0.0	5.500447	81116	81280	324-326	1.000000	42.7
rpl16-rps3_p5	rpl16-rps3	5	TGCGAAGTGATGAATTGGGT	CGAACTATTTCTGGGATATTAGGCA	20	25	45.0	40.0	57.8	58.5	0.0	0.0	5.734605	81116	81280	323-325	1.000000	42.7
rpl23-trnI (CAU)_p1	rpl23-trnI (CAU)	1	TTTCCCCAATAACCGACGAC	CCTGTTAAGCATCCATGGCT	20	20	50.0	50.0	57.9	57.9	0.0	7.0	0.160650	84607	85357	535-890	1.000000	87.3
rpl23-trnI (CAU)_p2	rpl23-trnI (CAU)	2	CCCCAATAACCGACGACTTT	CCTGTTAAGCATCCATGGCT	20	20	50.0	50.0	57.9	57.9	0.0	7.0	0.166508	84607	85357	532-887	1.000000	87.3
rpl23-trnI (CAU)_p3	rpl23-trnI (CAU)	3	AACCGACGACTTTTGTCTGT	CCTGTTAAGCATCCATGGCT	20	20	45.0	50.0	57.7	57.9	0.0	7.0	0.367722	84607	85357	525-880	1.000000	85.7
rpl23-trnI (CAU)_p4	rpl23-trnI (CAU)	4	TTTCCCCAATAACCGACGAC	ACGAGGATCCCTGTTAAGCA	20	20	50.0	50.0	57.9	58.4	0.0	8.6	0.529176	84607	85357	544-899	1.000000	84.3
rpl23-trnI (CAU)_p5	rpl23-trnI (CAU)	5	CCCCAATAACCGACGACTTT	ACGAGGATCCCTGTTAAGCA	20	20	50.0	50.0	57.9	58.4	0.0	8.6	0.535033	84607	85357	541-896	1.000000	84.3
trnI (CAU)-rpl23_p1	trnI (CAU)-rpl23	1	CCTGTTAAGCATCCATGGCT	TTTCCCCAATAACCGACGAC	20	20	50.0	50.0	57.9	57.9	7.0	0.0	0.160650	162475	163225	535-890	1.000000	87.3
trnI (CAU)-rpl23_p2	trnI (CAU)-rpl23	2	CCTGTTAAGCATCCATGGCT	CCCCAATAACCGACGACTTT	20	20	50.0	50.0	57.9	57.9	7.0	0.0	0.166508	162475	163225	532-887	1.000000	87.3
trnI (CAU)-rpl23_p3	trnI (CAU)-rpl23	3	CCTGTTAAGCATCCATGGCT	AACCGACGACTTTTGTCTGT	20	20	50.0	45.0	57.9	57.7	7.0	0.0	0.367722	162475	163225	525-880	1.000000	85.7
trnI (CAU)-rpl23_p4	trnI (CAU)-rpl23	4	ACGAGGATCCCTGTTAAGCA	TTTCCCCAATAACCGACGAC	20	20	50.0	50.0	58.4	57.9	8.6	0.0	0.529176	162475	163225	544-899	1.000000	84.3
trnI (CAU)-rpl23_p5	trnI (CAU)-rpl23	5	ACGAGGATCCCTGTTAAGCA	CCCCAATAACCGACGACTTT	20	20	50.0	50.0	58.4	57.9	8.6	0.0	0.535033	162475	163225	541-896	1.000000	84.3