Hotspot

rpoA

LSC · 75913-76920 · 1008 bp

Back to Cyananthus

Pi 0.00496

MarkerSeek score 61.60

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 5
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CGGTTGCAAGAAATTCAGTGG	CGCGTGTAGAAATCCAAACG	47.6	50.0	58.3	57.9	1114 bp	1.0	68.6
rpoA_p2	TCGGTTGCAAGAAATTCAGTG	CGCGTGTAGAAATCCAAACG	42.9	50.0	57.4	57.9	1115 bp	1.0	66.2
rpoA_p3	CGGTTGCAAGAAATTCAGTGG	AATGGCTGTAGACCTCCGAA	47.6	50.0	58.3	58.4	1141 bp	1.0	66.2
rpoA_p4	CGGTTGCAAGAAATTCAGTGG	ATGGCTGTAGACCTCCGAAA	47.6	50.0	58.3	58.4	1140 bp	1.0	66.2
rpoA_p5	CGGTTGCAAGAAATTCAGTGG	TAATGGCTGTAGACCTCCGA	47.6	50.0	58.3	57.6	1142 bp	1.0	66.1