Hotspot

rpl32-ndhF

SSC · 124729-125673 · 945 bp

Back to Cyananthus

Pi 0.0127

MarkerSeek score 66.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 945
variable_sites: 12
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ATGCCAAAGCTTTTAACGCC	CTGGAAGTGGAAAGAAGGGT	45.0	50.0	57.9	57.0	1095-1097 bp	1.0	72.7
rpl32-ndhF_p2	GAGCCCTTCCCTTTCCAAAT	CTGGAAGTGGAAAGAAGGGT	50.0	50.0	57.8	57.0	1069-1071 bp	1.0	71.5
rpl32-ndhF_p3	CAAAGCTTTTAACGCCGACC	CTGGAAGTGGAAAGAAGGGT	50.0	50.0	58.3	57.0	1091-1093 bp	1.0	70.8
rpl32-ndhF_p4	CCAAAGCTTTTAACGCCGAC	CTGGAAGTGGAAAGAAGGGT	50.0	50.0	58.3	57.0	1092-1094 bp	1.0	70.8
rpl32-ndhF_p5	AGCCCTTCCCTTTCCAAATT	CTGGAAGTGGAAAGAAGGGT	45.0	50.0	57.3	57.0	1068-1070 bp	1.0	67.6