Hotspot

trnL(UAG)-rpl32

SSC · 123712-124566 · 855 bp

Back to Cyananthus

Pi 0.0119
MarkerSeek score 64.90
Primer available yes
Label trnL(UAG)-rpl32

Feature Metadata

label_name
trnL(UAG)-rpl32
feature_type
igs
region
SSC
length_bp
855
variable_sites
10
pi
0.0119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL(UAG)-rpl32_p1 AGCGTGTCTACCGATTTCAC GGCGTTAAAAGCTTTGGCAT 50.0 45.0 58.0 57.9 966-969 bp 1.0 81.2
trnL(UAG)-rpl32_p2 CAGCGTGTCTACCGATTTCA GGCGTTAAAAGCTTTGGCAT 50.0 45.0 58.0 57.9 967-970 bp 1.0 81.2
trnL(UAG)-rpl32_p3 TCCTAAGAGCAGCGTGTCTA GGCGTTAAAAGCTTTGGCAT 50.0 45.0 57.9 57.9 976-979 bp 1.0 80.3
trnL(UAG)-rpl32_p4 AGCGTGTCTACCGATTTCAC ATTTGGAAAGGGAAGGGCTC 50.0 50.0 58.0 57.8 992-995 bp 1.0 79.9
trnL(UAG)-rpl32_p5 CAGCGTGTCTACCGATTTCA ATTTGGAAAGGGAAGGGCTC 50.0 50.0 58.0 57.8 993-996 bp 1.0 79.9