Hotspot

clpP-petA

LSC · 63505-64847 · 1343 bp

Back to Cyananthus

Pi 0.0204
MarkerSeek score 71.90
Primer available yes
Label clpP-petA

Feature Metadata

label_name
clpP-petA
feature_type
igs
region
LSC
length_bp
1343
variable_sites
27
pi
0.0204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-petA_p1 CCTTCTCCGATTCTGGCTTC CTTTTGCTCTTCTTGGCAGC 55.0 50.0 58.1 57.9 1509-1524 bp 1.0 81.6
clpP-petA_p2 CCTTCTCCGATTCTGGCTTC GCTCTTCTTGGCAGCTGTTA 55.0 50.0 58.1 58.2 1504-1519 bp 1.0 80.6
clpP-petA_p3 TCCTCCTTCTCCGATTCTGG CTTTTGCTCTTCTTGGCAGC 55.0 50.0 58.2 57.9 1513-1528 bp 1.0 80.2
clpP-petA_p4 ATCTCCGGTTTCTCCTCCTT CTTTTGCTCTTCTTGGCAGC 50.0 50.0 57.7 57.9 1525-1540 bp 1.0 79.9
clpP-petA_p5 CCTTCTCCGATTCTGGCTTC GGCAGCTGTTATTTTGGCAC 55.0 50.0 58.1 58.3 1495-1510 bp 1.0 79.8