Hotspot

psbK-trnQ(UUG)

LSC · 53883-54465 · 583 bp

Back to Cyananthus

Pi 0.0223
MarkerSeek score 56.40
Primer available yes
Label psbK-trnQ(UUG)

Feature Metadata

label_name
psbK-trnQ(UUG)
feature_type
igs
region
LSC
length_bp
583
variable_sites
13
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ(UUG)_p1 CGAAAACTTACAGCGGCTTG CTATTATCGCGCGGAACTGA 50.0 50.0 58.0 58.0 1392-1557 bp 1.0 83.5
psbK-trnQ(UUG)_p2 CGAAAACTTACAGCGGCTTG TCTATTATCGCGCGGAACTG 50.0 50.0 58.0 58.0 1393-1558 bp 1.0 83.5
psbK-trnQ(UUG)_p3 TTTTCGCCGCTATCGATGAA CTATTATCGCGCGGAACTGA 45.0 50.0 58.1 58.0 1515-1680 bp 1.0 83.0
psbK-trnQ(UUG)_p4 TTTTCGCCGCTATCGATGAA TCTATTATCGCGCGGAACTG 45.0 50.0 58.1 58.0 1516-1681 bp 1.0 83.0
psbK-trnQ(UUG)_p5 TTTTTCGCCGCTATCGATGA CTATTATCGCGCGGAACTGA 45.0 50.0 58.1 58.0 1516-1681 bp 1.0 83.0