Hotspot

psaA-ycf3

LSC · 18651-19380 · 730 bp

Back to Cyananthus

Pi 0.00822

MarkerSeek score 63.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 6
pi: 0.00822
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCCGGTCTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	927 bp	1.0	79.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	908 bp	1.0	79.3
psaA-ycf3_p3	TGAAAAATGACCCGGTCTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	929 bp	1.0	78.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	910 bp	1.0	78.5
psaA-ycf3_p5	TGAAAAATGACCCGGTCTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	856 bp	1.0	78.5