Hotspot

trnE(UUC)-trnT(GGU)

LSC · 6451-8406 · 1956 bp

Back to Cyananthus

Pi 0.0136

MarkerSeek score 57.60

Primer available yes

Label trnE(UUC)-trnT(GGU)

Feature Metadata

label_name: trnE(UUC)-trnT(GGU)
feature_type: igs
region: LSC
length_bp: 1956
variable_sites: 24
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE(UUC)-trnT(GGU)_p1	CGTTGCCTCCTTGAAAGAGA	GGACTTACGCCTTACCATGG	50.0	55.0	57.8	58.1	1869-2054 bp	1.0	83.2
trnE(UUC)-trnT(GGU)_p2	TGTCCTGAACCACTAGACGA	GGACTTACGCCTTACCATGG	50.0	55.0	57.7	58.1	1847-2032 bp	1.0	82.3
trnE(UUC)-trnT(GGU)_p3	CGTTGCCTCCTTGAAAGAGA	ACTTACGCCTTACCATGGTG	50.0	50.0	57.8	57.6	1867-2052 bp	1.0	80.4
trnE(UUC)-trnT(GGU)_p4	CCTGAACCACTAGACGATGG	GGACTTACGCCTTACCATGG	55.0	55.0	57.4	58.1	1844-2029 bp	1.0	79.9
trnE(UUC)-trnT(GGU)_p5	CTGAACCACTAGACGATGGG	GGACTTACGCCTTACCATGG	55.0	55.0	57.4	58.1	1843-2028 bp	1.0	79.9