Hotspot

trnH(GUG)-psbA

LSC · 78-414 · 337 bp

Back to Cyananthus

Pi 0.0148
MarkerSeek score 61.80
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
337
variable_sites
5
pi
0.0148
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 TCACAATCCACTGCCTTGTC TAACCGGGCTAACCTTGGTA 50.0 50.0 58.1 58.1 475 bp 1.0 79.6
trnH(GUG)-psbA_p2 TTCACAATCCACTGCCTTGT TAACCGGGCTAACCTTGGTA 45.0 50.0 57.6 58.1 476 bp 1.0 77.5
trnH(GUG)-psbA_p3 TCACAATCCACTGCCTTGTC ATTAACCGGGCTAACCTTGG 50.0 50.0 58.1 57.6 477 bp 1.0 76.6
trnH(GUG)-psbA_p4 TTGTCCACTTGGCTACATCC TAACCGGGCTAACCTTGGTA 50.0 50.0 57.5 58.1 460 bp 1.0 76.5
trnH(GUG)-psbA_p5 CCTTGTCCACTTGGCTACAT TAACCGGGCTAACCTTGGTA 50.0 50.0 57.5 58.1 462 bp 1.0 76.5