primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH(GUG)-psbA_p1	trnH(GUG)-psbA	1	TCACAATCCACTGCCTTGTC	TAACCGGGCTAACCTTGGTA	20	20	50.0	50.0	58.1	58.1	0.0	6.3	0.153981	78	414	475-475	1.000000	79.6
trnH(GUG)-psbA_p2	trnH(GUG)-psbA	2	TTCACAATCCACTGCCTTGT	TAACCGGGCTAACCTTGGTA	20	20	45.0	50.0	57.6	58.1	0.0	6.3	0.413507	78	414	476-476	1.000000	77.5
trnH(GUG)-psbA_p3	trnH(GUG)-psbA	3	TCACAATCCACTGCCTTGTC	ATTAACCGGGCTAACCTTGG	20	20	50.0	50.0	58.1	57.6	0.0	6.3	0.534028	78	414	477-477	1.000000	76.6
trnH(GUG)-psbA_p4	trnH(GUG)-psbA	4	TTGTCCACTTGGCTACATCC	TAACCGGGCTAACCTTGGTA	20	20	50.0	50.0	57.5	58.1	0.0	6.3	0.540139	78	414	460-460	1.000000	76.5
trnH(GUG)-psbA_p5	trnH(GUG)-psbA	5	CCTTGTCCACTTGGCTACAT	TAACCGGGCTAACCTTGGTA	20	20	50.0	50.0	57.5	58.1	0.0	6.3	0.547099	78	414	462-462	1.000000	76.5
matK-trnK(UUU)_p1	matK-trnK(UUU)	1	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3547	4274	859-859	1.000000	73.0
matK-trnK(UUU)_p2	matK-trnK(UUU)	2	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3547	4274	858-858	1.000000	73.0
matK-trnK(UUU)_p3	matK-trnK(UUU)	3	CCATGATCATGAGCAAGTGC	GGGTTTGATTGCCTCGTCTA	20	20	50.0	50.0	57.2	57.6	32.7	0.0	1.172901	3547	4274	941-941	1.000000	71.1
matK-trnK(UUU)_p4	matK-trnK(UUU)	4	CCATGATCATGAGCAAGTGC	TAGGGTTTGATTGCCTCGTC	20	20	50.0	50.0	57.2	57.6	32.7	0.0	1.172901	3547	4274	943-943	1.000000	71.1
matK-trnK(UUU)_p5	matK-trnK(UUU)	5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.2	57.5	32.7	0.0	1.237069	3547	4274	872-872	1.000000	70.7
rps16-trnD(GUC)_p1	rps16-trnD(GUC)	1	TTCCTTCTCTTCGGGATCGA	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.9	58.5	0.0	5.4	0.616655	5110	6060	1099-1106	1.000000	76.3
rps16-trnD(GUC)_p2	rps16-trnD(GUC)	2	CGGTGCTTTTTACCACATCG	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.8	58.5	0.0	5.4	0.718591	5110	6060	1049-1056	1.000000	75.5
rps16-trnD(GUC)_p3	rps16-trnD(GUC)	3	CGGGATCGAACATCAATTGC	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.6	58.5	0.0	5.4	0.867045	5110	6060	1088-1095	1.000000	74.3
rps16-trnD(GUC)_p4	rps16-trnD(GUC)	4	ATAAGTCGCACGGTGCTTTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	58.5	58.5	28.4	5.4	0.957923	5110	6060	1059-1066	1.000000	73.6
rps16-trnD(GUC)_p5	rps16-trnD(GUC)	5	CTTCCTTCTCTTCGGGATCG	TTCAATTGGTCAGAGCACCG	20	20	55.0	50.0	57.5	58.5	0.0	5.4	0.977840	5110	6060	1100-1107	1.000000	73.4
trnE(UUC)-trnT(GGU)_p1	trnE(UUC)-trnT(GGU)	1	CGTTGCCTCCTTGAAAGAGA	GGACTTACGCCTTACCATGG	20	20	50.0	55.0	57.8	58.1	0.0	11.2	0.220367	6451	8406	1869-2054	1.000000	83.2
trnE(UUC)-trnT(GGU)_p2	trnE(UUC)-trnT(GGU)	2	TGTCCTGAACCACTAGACGA	GGACTTACGCCTTACCATGG	20	20	50.0	55.0	57.7	58.1	0.0	11.2	0.332074	6451	8406	1847-2032	1.000000	82.3
trnE(UUC)-trnT(GGU)_p3	trnE(UUC)-trnT(GGU)	3	CGTTGCCTCCTTGAAAGAGA	ACTTACGCCTTACCATGGTG	20	20	50.0	50.0	57.8	57.6	0.0	28.2	0.566422	6451	8406	1867-2052	1.000000	80.4
trnE(UUC)-trnT(GGU)_p4	trnE(UUC)-trnT(GGU)	4	CCTGAACCACTAGACGATGG	GGACTTACGCCTTACCATGG	20	20	55.0	55.0	57.4	58.1	0.0	11.2	0.636793	6451	8406	1844-2029	1.000000	79.9
trnE(UUC)-trnT(GGU)_p5	trnE(UUC)-trnT(GGU)	5	CTGAACCACTAGACGATGGG	GGACTTACGCCTTACCATGG	20	20	55.0	55.0	57.4	58.1	0.0	11.2	0.636793	6451	8406	1843-2028	1.000000	79.9
psaA-ycf3_p1	psaA-ycf3	1	TGAAAAATGACCCGGTCTGG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.1	58.0	18.5	0.0	0.136672	18651	19380	927-927	1.000000	79.4
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	18651	19380	908-908	1.000000	79.3
psaA-ycf3_p3	psaA-ycf3	3	TGAAAAATGACCCGGTCTGG	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	58.1	58.1	18.5	0.0	0.237622	18651	19380	929-929	1.000000	78.6
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	18651	19380	910-910	1.000000	78.5
psaA-ycf3_p5	psaA-ycf3	5	TGAAAAATGACCCGGTCTGG	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.1	57.8	18.5	0.0	0.259531	18651	19380	856-856	1.000000	78.5
trnT(UGU)-psbM_p1	trnT(UGU)-psbM	1	AAGCCTACTCTCGGAATCGA	AAAATGAACAGTGCCGTAGC	20	20	50.0	45.0	57.9	57.0	0.0	0.0	1.043655	23592	24635	1156-1165	1.000000	75.0
trnT(UGU)-psbM_p2	trnT(UGU)-psbM	2	AGCCTACTCTCGGAATCGAA	AAAATGAACAGTGCCGTAGC	20	20	50.0	45.0	57.9	57.0	0.0	0.0	1.043655	23592	24635	1155-1164	1.000000	75.0
trnT(UGU)-psbM_p3	trnT(UGU)-psbM	3	CGATGACCTCCGCATTACAA	AAAATGAACAGTGCCGTAGC	20	20	50.0	45.0	58.1	57.0	0.0	0.0	1.054834	23592	24635	1134-1143	1.000000	74.9
trnT(UGU)-psbM_p4	trnT(UGU)-psbM	4	GCCTACTCTCGGAATCGAAC	AAAATGAACAGTGCCGTAGC	20	20	55.0	45.0	57.9	57.0	0.0	0.0	1.111985	23592	24635	1154-1163	1.000000	74.4
trnT(UGU)-psbM_p5	trnT(UGU)-psbM	5	GAATCGAACCGATGACCTCC	AAAATGAACAGTGCCGTAGC	20	20	55.0	45.0	58.1	57.0	2.4	0.0	1.135196	23592	24635	1143-1152	1.000000	74.3
trnR(UCU)-trnG(UCC)_p1	trnR(UCU)-trnG(UCC)	1	AGGGAAAAATTGCGTCCAAT	AGCCTTCCAAGCTAACGATG	20	20	40.0	50.0	56.2	58.0	5.1	0.0	1.844133	51284	51450	293-293	1.000000	66.1
trnR(UCU)-trnG(UCC)_p2	trnR(UCU)-trnG(UCC)	2	AGGGAAAAATTGCGTCCAAT	CCTAGCCTTCCAAGCTAACG	20	20	40.0	55.0	56.2	58.1	5.1	0.0	1.872970	51284	51450	296-296	1.000000	65.8
trnR(UCU)-trnG(UCC)_p3	trnR(UCU)-trnG(UCC)	3	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.081443	51284	51450	247-247	1.000000	64.3
trnR(UCU)-trnG(UCC)_p4	trnR(UCU)-trnG(UCC)	4	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	21	20	47.6	55.0	56.9	58.1	0.0	0.0	2.110280	51284	51450	250-250	1.000000	64.1
trnR(UCU)-trnG(UCC)_p5	trnR(UCU)-trnG(UCC)	5	AGGGAAAAATTGCGTCCAAT	CCCTAGCCTTCCAAGCTAAC	20	20	40.0	55.0	56.2	57.7	5.1	0.0	2.147983	51284	51450	297-297	1.000000	63.6
psbK-trnQ(UUG)_p1	psbK-trnQ(UUG)	1	CGAAAACTTACAGCGGCTTG	CTATTATCGCGCGGAACTGA	20	20	50.0	50.0	58.0	58.0	0.0	31.7	0.095811	53883	54465	1392-1557	1.000000	83.5
psbK-trnQ(UUG)_p2	psbK-trnQ(UUG)	2	CGAAAACTTACAGCGGCTTG	TCTATTATCGCGCGGAACTG	20	20	50.0	50.0	58.0	58.0	0.0	31.7	0.095811	53883	54465	1393-1558	1.000000	83.5
psbK-trnQ(UUG)_p3	psbK-trnQ(UUG)	3	TTTTCGCCGCTATCGATGAA	CTATTATCGCGCGGAACTGA	20	20	45.0	50.0	58.1	58.0	8.5	31.7	0.117052	53883	54465	1515-1680	1.000000	83.0
psbK-trnQ(UUG)_p4	psbK-trnQ(UUG)	4	TTTTCGCCGCTATCGATGAA	TCTATTATCGCGCGGAACTG	20	20	45.0	50.0	58.1	58.0	8.5	31.7	0.117052	53883	54465	1516-1681	1.000000	83.0
psbK-trnQ(UUG)_p5	psbK-trnQ(UUG)	5	TTTTTCGCCGCTATCGATGA	CTATTATCGCGCGGAACTGA	20	20	45.0	50.0	58.1	58.0	8.5	31.7	0.117052	53883	54465	1516-1681	1.000000	83.0
trnQ(UUG)_p1	trnQ(UUG)	1	AAAGAAATTCCCCGCGCTAT	CTATTATCGCGCGGAACTGA	20	20	45.0	50.0	57.9	58.0	5.6	31.7	0.099065	54466	54538	888-1053	1.000000	85.5
trnQ(UUG)_p2	trnQ(UUG)	2	AAAGAAATTCCCCGCGCTAT	TCTATTATCGCGCGGAACTG	20	20	45.0	50.0	57.9	58.0	5.6	31.7	0.099065	54466	54538	889-1054	1.000000	85.5
trnQ(UUG)_p3	trnQ(UUG)	3	AAAGAAATTCCCCGCGCTAT	CTAGAGTCAGGCCAAGACCT	20	20	45.0	55.0	57.9	58.1	5.6	20.8	0.193480	54466	54538	814-979	1.000000	85.2
trnQ(UUG)_p4	trnQ(UUG)	4	AAAGAAATTCCCCGCGCTAT	TCTAGAGTCAGGCCAAGACC	20	20	45.0	55.0	57.9	58.1	5.6	20.8	0.198819	54466	54538	815-980	1.000000	85.2
trnQ(UUG)_p5	trnQ(UUG)	5	CGAAAACTTACAGCGGCTTG	AGCAACGCATTTTGAATCCG	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.068275	54466	54538	1795-1960	1.000000	82.9
clpP-petA_p1	clpP-petA	1	CCTTCTCCGATTCTGGCTTC	CTTTTGCTCTTCTTGGCAGC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.114995	63505	64847	1509-1524	1.000000	81.6
clpP-petA_p2	clpP-petA	2	CCTTCTCCGATTCTGGCTTC	GCTCTTCTTGGCAGCTGTTA	20	20	55.0	50.0	58.1	58.2	0.0	16.6	0.239742	63505	64847	1504-1519	1.000000	80.6
clpP-petA_p3	clpP-petA	3	TCCTCCTTCTCCGATTCTGG	CTTTTGCTCTTCTTGGCAGC	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.279954	63505	64847	1513-1528	1.000000	80.2
clpP-petA_p4	clpP-petA	4	ATCTCCGGTTTCTCCTCCTT	CTTTTGCTCTTCTTGGCAGC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.317510	63505	64847	1525-1540	1.000000	79.9
clpP-petA_p5	clpP-petA	5	CCTTCTCCGATTCTGGCTTC	GGCAGCTGTTATTTTGGCAC	20	20	55.0	50.0	58.1	58.3	0.0	16.6	0.339658	63505	64847	1495-1510	1.000000	79.8
rpoA_p1	rpoA	1	CGGTTGCAAGAAATTCAGTGG	CGCGTGTAGAAATCCAAACG	21	20	47.6	50.0	58.3	57.9	0.0	0.0	1.474420	75913	76920	1114-1114	1.000000	68.6
rpoA_p2	rpoA	2	TCGGTTGCAAGAAATTCAGTG	CGCGTGTAGAAATCCAAACG	21	20	42.9	50.0	57.4	57.9	0.0	0.0	1.763663	75913	76920	1115-1115	1.000000	66.2
rpoA_p3	rpoA	3	CGGTTGCAAGAAATTCAGTGG	AATGGCTGTAGACCTCCGAA	21	20	47.6	50.0	58.3	58.4	0.0	0.0	1.766580	75913	76920	1141-1141	1.000000	66.2
rpoA_p4	rpoA	4	CGGTTGCAAGAAATTCAGTGG	ATGGCTGTAGACCTCCGAAA	21	20	47.6	50.0	58.3	58.4	0.0	0.0	1.766580	75913	76920	1140-1140	1.000000	66.2
rpoA_p5	rpoA	5	CGGTTGCAAGAAATTCAGTGG	TAATGGCTGTAGACCTCCGA	21	20	47.6	50.0	58.3	57.6	0.0	0.0	1.782492	75913	76920	1142-1142	1.000000	66.1
trnN(GUU)-ycf1_p1	trnN(GUU)-ycf1	1	AAACAAAGACGGGAATGGCT	TCCGTATCCAGGCGACTAAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.013503	106030	106726	1392-2217	1.000000	82.5
trnN(GUU)-ycf1_p2	trnN(GUU)-ycf1	2	AACAAAGACGGGAATGGCTT	TCCGTATCCAGGCGACTAAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.013503	106030	106726	1391-2216	1.000000	82.5
trnN(GUU)-ycf1_p3	trnN(GUU)-ycf1	3	AAACAAAGACGGGAATGGCT	ACCAACAAAACTGCTCGCTA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042830	106030	106726	1331-2156	1.000000	82.3
trnN(GUU)-ycf1_p4	trnN(GUU)-ycf1	4	AACAAAGACGGGAATGGCTT	ACCAACAAAACTGCTCGCTA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042830	106030	106726	1330-2155	1.000000	82.3
trnN(GUU)-ycf1_p5	trnN(GUU)-ycf1	5	AACAAAGACGGGAATGGCTT	TACTGCTACCCTCTTCTCGG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.058856	106030	106726	1021-1846	1.000000	82.2
ycf1_p1	ycf1	1	ATGCCTGCAGGAAAGTTTCA	GGGTTTGGTGTGGATGAGTT	20	20	45.0	50.0	58.0	58.0	37.2	0.0	0.004976	110113	110232	864-966	1.000000	84.2
ycf1_p2	ycf1	2	ATGCCTGCAGGAAAGTTTCA	AGTCCCCAGAAAGCCCTTAT	20	20	45.0	50.0	58.0	58.0	37.2	0.0	0.013272	110113	110232	1283-1385	1.000000	83.4
ycf1_p3	ycf1	3	ATGCCTGCAGGAAAGTTTCA	ATAGTCCCCAGAAAGCCCTT	20	20	45.0	50.0	58.0	58.0	37.2	0.0	0.013272	110113	110232	1285-1387	1.000000	83.4
ycf1_p4	ycf1	4	ATGCCTGCAGGAAAGTTTCA	AAGGAAGGGTCGTGTAGGAA	20	20	45.0	50.0	58.0	58.0	37.2	0.0	0.020159	110113	110232	1325-1427	1.000000	83.2
ycf1_p5	ycf1	5	ATGCCTGCAGGAAAGTTTCA	AGGAAGGGTCGTGTAGGAAA	20	20	45.0	50.0	58.0	58.0	37.2	0.0	0.020159	110113	110232	1324-1426	1.000000	83.2
ndhD-ccsA_p1	ndhD-ccsA	1	CCTGTAATAGGCGTTGGGAT	CTGCAATTGTGGCGACTCTA	20	20	50.0	50.0	57.1	58.3	0.0	7.4	1.205186	122325	122561	449-449	1.000000	71.6
ndhD-ccsA_p2	ndhD-ccsA	2	CCTGTAATAGGCGTTGGGAT	TGCAATTGTGGCGACTCTAG	20	20	50.0	50.0	57.1	58.3	0.0	7.4	1.205186	122325	122561	448-448	1.000000	71.6
ndhD-ccsA_p3	ndhD-ccsA	3	CCTGTAATAGGCGTTGGGAT	AATTCTGCAATTGTGGCGAC	20	20	50.0	45.0	57.1	57.6	0.0	7.4	1.283329	122325	122561	453-453	1.000000	71.0
ndhD-ccsA_p4	ndhD-ccsA	4	CCTGTAATAGGCGTTGGGAT	ATTCTGCAATTGTGGCGACT	20	20	50.0	45.0	57.1	58.5	0.0	7.4	1.392779	122325	122561	452-452	1.000000	70.1
ndhD-ccsA_p5	ndhD-ccsA	5	ATAGGCGTTGGGATGTATCC	TGCAATTGTGGCGACTCTAG	20	20	50.0	50.0	56.8	58.3	2.2	7.4	1.426492	122325	122561	442-442	1.000000	69.9
trnL(UAG)-rpl32_p1	trnL(UAG)-rpl32	1	AGCGTGTCTACCGATTTCAC	GGCGTTAAAAGCTTTGGCAT	20	20	50.0	45.0	58.0	57.9	0.0	13.5	0.086679	123712	124566	966-969	1.000000	81.2
trnL(UAG)-rpl32_p2	trnL(UAG)-rpl32	2	CAGCGTGTCTACCGATTTCA	GGCGTTAAAAGCTTTGGCAT	20	20	50.0	45.0	58.0	57.9	0.0	13.5	0.088049	123712	124566	967-970	1.000000	81.2
trnL(UAG)-rpl32_p3	trnL(UAG)-rpl32	3	TCCTAAGAGCAGCGTGTCTA	GGCGTTAAAAGCTTTGGCAT	20	20	50.0	45.0	57.9	57.9	0.0	13.5	0.203126	123712	124566	976-979	1.000000	80.3
trnL(UAG)-rpl32_p4	trnL(UAG)-rpl32	4	AGCGTGTCTACCGATTTCAC	ATTTGGAAAGGGAAGGGCTC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.245914	123712	124566	992-995	1.000000	79.9
trnL(UAG)-rpl32_p5	trnL(UAG)-rpl32	5	CAGCGTGTCTACCGATTTCA	ATTTGGAAAGGGAAGGGCTC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.247285	123712	124566	993-996	1.000000	79.9
rpl32-ndhF_p1	rpl32-ndhF	1	ATGCCAAAGCTTTTAACGCC	CTGGAAGTGGAAAGAAGGGT	20	20	45.0	50.0	57.9	57.0	9.5	0.0	1.038314	124729	125673	1095-1097	1.000000	72.7
rpl32-ndhF_p2	rpl32-ndhF	2	GAGCCCTTCCCTTTCCAAAT	CTGGAAGTGGAAAGAAGGGT	20	20	50.0	50.0	57.8	57.0	0.0	0.0	1.197549	124729	125673	1069-1071	1.000000	71.5
rpl32-ndhF_p3	rpl32-ndhF	3	CAAAGCTTTTAACGCCGACC	CTGGAAGTGGAAAGAAGGGT	20	20	50.0	50.0	58.3	57.0	9.5	0.0	1.277640	124729	125673	1091-1093	1.000000	70.8
rpl32-ndhF_p4	rpl32-ndhF	4	CCAAAGCTTTTAACGCCGAC	CTGGAAGTGGAAAGAAGGGT	20	20	50.0	50.0	58.3	57.0	9.5	0.0	1.277640	124729	125673	1092-1094	1.000000	70.8
rpl32-ndhF_p5	rpl32-ndhF	5	AGCCCTTCCCTTTCCAAATT	CTGGAAGTGGAAAGAAGGGT	20	20	45.0	50.0	57.3	57.0	0.0	0.0	1.681050	124729	125673	1068-1070	1.000000	67.6
ycf1-trnN(GUU)_p1	ycf1-trnN(GUU)	1	TCCGTATCCAGGCGACTAAT	AAACAAAGACGGGAATGGCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.013503	141451	142147	1392-2217	1.000000	82.5
ycf1-trnN(GUU)_p2	ycf1-trnN(GUU)	2	TCCGTATCCAGGCGACTAAT	AACAAAGACGGGAATGGCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.013503	141451	142147	1391-2216	1.000000	82.5
ycf1-trnN(GUU)_p3	ycf1-trnN(GUU)	3	ACCAACAAAACTGCTCGCTA	AAACAAAGACGGGAATGGCT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042830	141451	142147	1331-2156	1.000000	82.3
ycf1-trnN(GUU)_p4	ycf1-trnN(GUU)	4	ACCAACAAAACTGCTCGCTA	AACAAAGACGGGAATGGCTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042830	141451	142147	1330-2155	1.000000	82.3
ycf1-trnN(GUU)_p5	ycf1-trnN(GUU)	5	TACTGCTACCCTCTTCTCGG	AACAAAGACGGGAATGGCTT	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.058856	141451	142147	1021-1846	1.000000	82.2