Hotspot

rpl2

LSC · 50077-50889 · 813 bp

Back to Cuscuta

Pi 0.113

MarkerSeek score 82.90

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 813
variable_sites: 342
pi: 0.113
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAACGACCCCTAATTCGGTC	ATTGGCGAAGTCGTAGGTTC	55.0	50.0	58.1	58.0	1546-1576 bp	0.157895	48.1
rpl2_p2	GAACGACCCCTAATTCGGTC	GGCGAAGTCGTAGGTTCAAT	55.0	50.0	58.1	58.0	1543-1573 bp	0.157895	48.1
rpl2_p3	GAACGACCCCTAATTCGGTC	GAGACGCGGATCAAAGAGTT	55.0	50.0	58.1	58.0	2032-2057 bp	0.157895	47.9
rpl2_p4	GAACGACCCCTAATTCGGTC	GTTGAACCCGGTAAATTGCG	55.0	50.0	58.1	58.0	2061-2086 bp	0.157895	47.7
rpl2_p5	GAACGACCCCTAATTCGGTC	TTGCGTGAAAGTAGGAGACG	55.0	50.0	58.1	57.9	2046-2071 bp	0.157895	47.5