Hotspot

rpl16

LSC · 47248-48475 · 1228 bp

Back to Cuscuta

Pi 0.162

MarkerSeek score 81.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1228
variable_sites: 647
pi: 0.162
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGCATATCGCCGATTGCTAG	GGCGGAAGAAGCAGATACAA	50.0	50.0	58.0	58.0	1587-1920 bp	0.368421	66.5
rpl16_p2	GCATATCGCCGATTGCTAGA	GGCGGAAGAAGCAGATACAA	50.0	50.0	58.0	58.0	1586-1919 bp	0.368421	66.5
rpl16_p3	GCTCTGACGACTTCCGATTT	GGCGGAAGAAGCAGATACAA	50.0	50.0	58.0	58.0	1803-2000 bp	0.315789	64.4
rpl16_p4	AGCATATCGCCGATTGCTAG	TTGGCGGAAGAAGCAGATAC	50.0	50.0	58.0	58.0	1725-1922 bp	0.263158	62.3
rpl16_p5	GCTCTGACGACTTCCGATTT	TTGGCGGAAGAAGCAGATAC	50.0	50.0	58.0	58.0	1805-2002 bp	0.263158	62.3