Hotspot

psbB

LSC · 39285-40811 · 1527 bp

Back to Cuscuta

Pi 0.0784

MarkerSeek score 81.50

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 491
pi: 0.0784
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TGAAAGGGGGCTATCTCGAT	CCGTCGCAACAACTAAGAGA	50.0	50.0	57.9	57.9	2200-2251 bp	0.368421	64.8
psbB_p2	ATTTTTGGTGAAAGGGGGCT	CCGTCGCAACAACTAAGAGA	45.0	50.0	57.9	57.9	2235-2259 bp	0.263158	56.4
psbB_p3	TGAAAGGGGGCTATCTCGAT	GTATTGTAGGTGCTTCGCGA	50.0	50.0	57.9	58.1	2080-2112 bp	0.157895	52.8
psbB_p4	TGAAAGGGGGCTATCTCGAT	TGGTATTGTAGGTGCTTCGC	50.0	50.0	57.9	58.0	2114 bp	0.105263	43.2
psbB_p5	ATTTTTGGTGAAAGGGGGCT	TGGTATTGTAGGTGCTTCGC	45.0	50.0	57.9	58.0	2122 bp	0.105263	43.1