Hotspot

psbA

LSC · 168-1229 · 1062 bp

Back to Cuscuta

Pi 0.0637
MarkerSeek score 80.40
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
271
pi
0.0637
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 CAATCCACTGCCTTCATCCA TCCTTCCGTCCCAGATCTTT 50.0 50.0 57.9 58.0 1446-1877 bp 0.263158 61.1
psbA_p2 AATCCACTGCCTTCATCCAC TCCTTCCGTCCCAGATCTTT 50.0 50.0 57.9 58.0 1445-1876 bp 0.263158 61.1
psbA_p3 ACAATCCACTGCCTTCATCC TCCTTCCGTCCCAGATCTTT 50.0 50.0 57.9 58.0 1447-1878 bp 0.263158 61.1
psbA_p4 CAATCCACTGCCTTCATCCA TTTTGGATGGGAAAACGGGT 50.0 45.0 57.9 57.9 1717-1722 bp 0.157895 47.7
psbA_p5 AATCCACTGCCTTCATCCAC TTTTGGATGGGAAAACGGGT 50.0 45.0 57.9 57.9 1716-1721 bp 0.157895 47.7