Hotspot

accD

LSC · 28016-29494 · 1479 bp

Back to Cuscuta

Pi 0.136

MarkerSeek score 83.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 753
pi: 0.136
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGGGCTTCTACTTTCAGCAC	CCAGGAAAGACCCAACGAAA	50.0	50.0	57.8	58.0	2402-2637 bp	0.210526	58.7
accD_p2	TGGGCTTCTACTTTCAGCAC	CTGTTCTGATCGCCAACTCA	50.0	50.0	57.8	57.9	2115-2350 bp	0.210526	58.3
accD_p3	ATGCTTGCAGGCTAATGTGT	CCAGGAAAGACCCAACGAAA	45.0	50.0	58.2	58.0	2777-2782 bp	0.157895	48.9
accD_p4	ATGCTTGCAGGCTAATGTGT	CTGTTCTGATCGCCAACTCA	45.0	50.0	58.2	57.9	2490-2495 bp	0.157895	48.5
accD_p5	ATGCTTGCAGGCTAATGTGT	ACTTCCTCTTCTAGACCCCG	45.0	55.0	58.2	57.9	2529-2534 bp	0.157895	47.9