Hotspot

petD-rps11

LSC · 44336-45350 · 1015 bp

Back to Cuscuta

Pi 0.312

MarkerSeek score 72.40

Primer available yes

Label petD-rps11

Feature Metadata

label_name: petD-rps11
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 762
pi: 0.312
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rps11_p1	CAAAATCCATTTCGCCGTCC	TGGAAGAGACGCAGCATTAC	50.0	50.0	58.1	58.0	668-1256 bp	0.578947	74.3
petD-rps11_p2	GGTTTCAATACCTGCGGGAT	CACAATGGCTGTAGACCTCC	50.0	55.0	57.9	58.0	647-1256 bp	0.526316	72.4
petD-rps11_p3	GGTTTCAATACCTGCGGGAT	TGGAAGAGACGCAGCATTAC	50.0	50.0	57.9	58.0	726-1314 bp	0.421053	68.3
petD-rps11_p4	TTCGCCGTCCAGTATCTACT	CACAATGGCTGTAGACCTCC	50.0	55.0	58.0	58.0	579-1188 bp	0.368421	66.1
petD-rps11_p5	TTCGCCGTCCAGTATCTACT	TGGAAGAGACGCAGCATTAC	50.0	50.0	58.0	58.0	658-1246 bp	0.315789	64.2