Hotspot

rbcL-accD

LSC · 26931-28015 · 1085 bp

Back to Cuscuta

Pi 0.232

MarkerSeek score 77.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 708
pi: 0.232
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GGCTTCAGGGGGTATTCATG	GGATTCCACGTCCCTTCATC	55.0	55.0	58.0	58.0	1478-2238 bp	0.578947	74.7
rbcL-accD_p2	TACAAGCTCGTAACGAAGGC	GGATTCCACGTCCCTTCATC	50.0	55.0	58.0	58.0	1746-1794 bp	0.157895	51.1
rbcL-accD_p3	GCTCGTAACGAAGGCCTTAA	GGATTCCACGTCCCTTCATC	50.0	55.0	58.0	58.0	1788-1789 bp	0.105263	43.9
rbcL-accD_p4	GCTCGTAACGAAGGCCTTAA	TCTAGAGGATTCCACGTCCC	50.0	55.0	58.0	57.9	1794-1795 bp	0.105263	43.7
rbcL-accD_p5	GCTCGTAACGAAGGCCTTAA	CTAGAGGATTCCACGTCCCT	50.0	55.0	58.0	57.9	1793-1794 bp	0.105263	43.7