Hotspot

psbA-trnQ-UUG

LSC · 1230-1896 · 667 bp

Back to Cuscuta

Pi 0.287

MarkerSeek score 73.80

Primer available yes

Label psbA-trnQ-UUG

Feature Metadata

label_name: psbA-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 667
variable_sites: 478
pi: 0.287
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnQ-UUG_p1	GGAGCCTCCAAATACACCAG	CTTCGGGCAATTTGCTGAAG	55.0	50.0	58.0	58.0	1347-1787 bp	0.315789	64.2
psbA-trnQ-UUG_p2	CAGAGATTCCCAAGGGCATC	CTTCGGGCAATTTGCTGAAG	55.0	50.0	58.0	58.0	1682-1712 bp	0.263158	62.2
psbA-trnQ-UUG_p3	GCATACCCAAGCGGAAACTA	CTTCGGGCAATTTGCTGAAG	50.0	50.0	58.0	58.0	1138-1578 bp	0.263158	62.2
psbA-trnQ-UUG_p4	AAGTGCAACCCTATAGCTGC	CTTCGGGCAATTTGCTGAAG	50.0	50.0	58.0	58.0	998-1438 bp	0.210526	60.0
psbA-trnQ-UUG_p5	CTCCAAATACACCAGCGACA	CTTCGGGCAATTTGCTGAAG	50.0	50.0	57.9	58.0	1772-1782 bp	0.210526	59.6