primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbA_p1 psbA 1 CAATCCACTGCCTTCATCCA TCCTTCCGTCCCAGATCTTT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.180660 168 1229 1446-1877 0.263158 61.1 psbA_p2 psbA 2 AATCCACTGCCTTCATCCAC TCCTTCCGTCCCAGATCTTT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.182168 168 1229 1445-1876 0.263158 61.1 psbA_p3 psbA 3 ACAATCCACTGCCTTCATCC TCCTTCCGTCCCAGATCTTT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.182168 168 1229 1447-1878 0.263158 61.1 psbA_p4 psbA 4 CAATCCACTGCCTTCATCCA TTTTGGATGGGAAAACGGGT 20 20 50.0 45.0 57.9 57.9 0.0 0.0 0.222882 168 1229 1717-1722 0.157895 47.7 psbA_p5 psbA 5 AATCCACTGCCTTCATCCAC TTTTGGATGGGAAAACGGGT 20 20 50.0 45.0 57.9 57.9 0.0 0.0 0.224389 168 1229 1716-1721 0.157895 47.7 psbA-trnQ-UUG_p1 psbA-trnQ-UUG 1 GGAGCCTCCAAATACACCAG CTTCGGGCAATTTGCTGAAG 20 20 55.0 50.0 58.0 58.0 5.7 26.8 0.057425 1230 1896 1347-1787 0.315789 64.2 psbA-trnQ-UUG_p2 psbA-trnQ-UUG 2 CAGAGATTCCCAAGGGCATC CTTCGGGCAATTTGCTGAAG 20 20 55.0 50.0 58.0 58.0 9.4 26.8 0.034848 1230 1896 1682-1712 0.263158 62.2 psbA-trnQ-UUG_p3 psbA-trnQ-UUG 3 GCATACCCAAGCGGAAACTA CTTCGGGCAATTTGCTGAAG 20 20 50.0 50.0 58.0 58.0 0.0 26.8 0.045983 1230 1896 1138-1578 0.263158 62.2 psbA-trnQ-UUG_p4 psbA-trnQ-UUG 4 AAGTGCAACCCTATAGCTGC CTTCGGGCAATTTGCTGAAG 20 20 50.0 50.0 58.0 58.0 0.0 26.8 0.057425 1230 1896 998-1438 0.210526 60.0 psbA-trnQ-UUG_p5 psbA-trnQ-UUG 5 CTCCAAATACACCAGCGACA CTTCGGGCAATTTGCTGAAG 20 20 50.0 50.0 57.9 58.0 0.0 26.8 0.106608 1230 1896 1772-1782 0.210526 59.6 rps2_p1 rps2 1 CCCAAGACGCCATAAGTACC TTGGCTAAGTCCTGTCAGGA 20 20 55.0 50.0 58.1 58.0 0.0 18.1 0.074710 7049 7759 1523-1523 0.105263 43.6 rps2_p2 rps2 2 TCGACACTCGCTAGCTCATA TTGGCTAAGTCCTGTCAGGA 20 20 50.0 50.0 58.1 58.0 13.9 18.1 0.074710 7049 7759 1452-1452 0.105263 43.6 rps2_p3 rps2 3 AGGGACCATAACCTTCACCA TTGGCTAAGTCCTGTCAGGA 20 20 50.0 50.0 57.9 58.0 0.0 18.1 0.077923 7049 7759 1670-1670 0.105263 43.6 rps2_p4 rps2 4 AAAGGAACCCAGGGACCATA TTGGCTAAGTCCTGTCAGGA 20 20 50.0 50.0 57.9 58.0 14.5 18.1 0.093007 7049 7759 1680-1680 0.105263 43.5 rps2_p5 rps2 5 AAGGAACCCAGGGACCATAA TTGGCTAAGTCCTGTCAGGA 20 20 50.0 50.0 57.9 58.0 14.5 18.1 0.093007 7049 7759 1679-1679 0.105263 43.5 rps2-trnC-GCA_p1 rps2-trnC-GCA 1 CTTGTTTCTGTGGTGGACCA GCAGCACTTCTAGAGTCCAC 20 20 50.0 55.0 57.9 58.0 0.0 0.0 0.054531 7760 8987 1348-2185 0.578947 74.7 rps2-trnC-GCA_p2 rps2-trnC-GCA 2 CCCTGACAAATCGCGAAAAC GCAGCACTTCTAGAGTCCAC 20 20 50.0 55.0 58.0 58.0 15.2 0.0 0.039450 7760 8987 2434-2468 0.157895 51.7 rps2-trnC-GCA_p3 rps2-trnC-GCA 3 CCTGACAAATCGCGAAAACC GCAGCACTTCTAGAGTCCAC 20 20 50.0 55.0 58.0 58.0 15.2 0.0 0.039450 7760 8987 2433-2467 0.157895 51.7 rps2-trnC-GCA_p4 rps2-trnC-GCA 4 CCCTGACAAATCGCGAAAAC AGAATTGATCAGGCGACACC 20 20 50.0 50.0 58.0 58.0 15.2 0.0 0.066091 7760 8987 1993-1993 0.105263 43.7 rps2-trnC-GCA_p5 rps2-trnC-GCA 5 CCTGACAAATCGCGAAAACC AGAATTGATCAGGCGACACC 20 20 50.0 50.0 58.0 58.0 15.2 0.0 0.066091 7760 8987 1992-1992 0.105263 43.7 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GTAACACCATGGTAAGGCGT TTGACCAAGGGTTATAGTCA 20 20 50.0 40.0 57.9 53.2 19.3 0.0 4.862837 10767 11436 553-786 0.789474 44.7 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GTAACACCATGGTAAGGCGT TGACCAAGGGTTATAGTCAT 20 20 50.0 40.0 57.9 53.0 19.3 0.0 5.112938 10767 11436 552-785 0.789474 43.6 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 GTAACACCATGGTAAGGCGT GATTGACCAAGGGTTATAGTCA 20 22 50.0 40.9 57.9 55.2 19.3 0.0 4.854365 10767 11436 555-743 0.736842 42.6 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 ACACCATGGTAAGGCGTAAG GATTGACCAAGGGTTATAGTCA 20 22 50.0 40.9 57.6 55.2 19.3 0.0 5.147418 10767 11436 552-740 0.684211 39.4 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 CACCATGGTAAGGCGTAAGT GATTGACCAAGGGTTATAGTCA 20 22 50.0 40.9 57.6 55.2 19.3 0.0 5.147418 10767 11436 551-739 0.684211 39.4 psbD_p1 psbD 1 GCGGTAGAGTAACACCATGG GACAATCCATCCGTCTCCAC 20 20 55.0 55.0 58.1 58.1 11.2 0.0 0.122742 11437 12498 2235-2457 0.578947 74.2 psbD_p2 psbD 2 GCGGTAGAGTAACACCATGG CTAGAGTCGCTAAGTGGGGA 20 20 55.0 55.0 58.1 57.9 11.2 0.0 0.118300 11437 12498 1846-2023 0.368421 65.8 psbD_p3 psbD 3 GCGGTAGAGTAACACCATGG CACCCCCAAAATAAAGGGCT 20 20 55.0 50.0 58.1 58.0 11.2 0.0 0.112858 11437 12498 2290-2290 0.105263 43.3 psbD_p4 psbD 4 TCAGCGGTAGAGTAACACCA CACCCCCAAAATAAAGGGCT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.135013 11437 12498 2293-2293 0.105263 43.1 psbD_p5 psbD 5 GCGTCAAGCGACTATTCTCA CACCCCCAAAATAAAGGGCT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.139415 11437 12498 2034-2034 0.105263 43.1 psbC_p1 psbC 1 GCCTTTAACCCAACGCAATC AATCGAACCCGCATCTTCTC 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.067876 12446 13867 2441-2553 0.578947 74.6 psbC_p2 psbC 2 GCCTTTAACCCAACGCAATC GAATCGAACCCGCATCTTCT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.067876 12446 13867 2442-2554 0.578947 74.6 psbC_p3 psbC 3 GATGGGAGTTTCCGGTGTAC AATCGAACCCGCATCTTCTC 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.078623 12446 13867 1465-2658 0.578947 74.5 psbC_p4 psbC 4 TTTCCCAAGAAATTCGGGCA AATCGAACCCGCATCTTCTC 20 20 45.0 50.0 58.0 58.1 1.6 0.0 0.061180 12446 13867 2249-2357 0.263158 62.0 psbC_p5 psbC 5 TTTCCCAAGAAATTCGGGCA GAATCGAACCCGCATCTTCT 20 20 45.0 50.0 58.0 58.1 1.6 0.0 0.061180 12446 13867 2250-2358 0.263158 62.0 rbcL_p1 rbcL 1 ACCGACCGGAACACTTATTG GTGCTGAAAGTAGAAGCCCA 20 20 50.0 50.0 57.9 57.8 16.1 0.0 0.273954 25464 26930 2500-2805 0.210526 57.7 rbcL_p2 rbcL 2 CGTCCTTGAACTACCAGAGC ACACATTAGCCTGCAAGCAT 20 20 55.0 45.0 58.0 58.2 0.0 10.5 0.158963 25464 26930 2468-2512 0.157895 48.8 rbcL_p3 rbcL 3 CGTCCTTGAACTACCAGAGC GTGCTGAAAGTAGAAGCCCA 20 20 55.0 50.0 58.0 57.8 0.0 0.0 0.187335 25464 26930 2609-2657 0.157895 48.8 rbcL_p4 rbcL 4 ACCGACCGGAACACTTATTG ACACATTAGCCTGCAAGCAT 20 20 50.0 45.0 57.9 58.2 16.1 10.5 0.245581 25464 26930 2616-2660 0.157895 47.9 rbcL_p5 rbcL 5 CACCGACCGGAACACTTATT ACACATTAGCCTGCAAGCAT 20 20 50.0 45.0 57.9 58.2 16.1 10.5 0.245581 25464 26930 2617-2661 0.157895 47.9 rbcL-accD_p1 rbcL-accD 1 GGCTTCAGGGGGTATTCATG GGATTCCACGTCCCTTCATC 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.058191 26931 28015 1478-2238 0.578947 74.7 rbcL-accD_p2 rbcL-accD 2 TACAAGCTCGTAACGAAGGC GGATTCCACGTCCCTTCATC 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.058375 26931 28015 1746-1794 0.157895 51.1 rbcL-accD_p3 rbcL-accD 3 GCTCGTAACGAAGGCCTTAA GGATTCCACGTCCCTTCATC 20 20 50.0 55.0 58.0 58.0 20.5 0.0 0.045502 26931 28015 1788-1789 0.105263 43.9 rbcL-accD_p4 rbcL-accD 4 GCTCGTAACGAAGGCCTTAA TCTAGAGGATTCCACGTCCC 20 20 50.0 55.0 58.0 57.9 20.5 5.3 0.069263 26931 28015 1794-1795 0.105263 43.7 rbcL-accD_p5 rbcL-accD 5 GCTCGTAACGAAGGCCTTAA CTAGAGGATTCCACGTCCCT 20 20 50.0 55.0 58.0 57.9 20.5 5.3 0.075158 26931 28015 1793-1794 0.105263 43.7 accD_p1 accD 1 TGGGCTTCTACTTTCAGCAC CCAGGAAAGACCCAACGAAA 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.209344 28016 29494 2402-2637 0.210526 58.7 accD_p2 accD 2 TGGGCTTCTACTTTCAGCAC CTGTTCTGATCGCCAACTCA 20 20 50.0 50.0 57.8 57.9 0.0 3.7 0.269614 28016 29494 2115-2350 0.210526 58.3 accD_p3 accD 3 ATGCTTGCAGGCTAATGTGT CCAGGAAAGACCCAACGAAA 20 20 45.0 50.0 58.2 58.0 22.3 0.0 0.180972 28016 29494 2777-2782 0.157895 48.9 accD_p4 accD 4 ATGCTTGCAGGCTAATGTGT CTGTTCTGATCGCCAACTCA 20 20 45.0 50.0 58.2 57.9 22.3 3.7 0.241241 28016 29494 2490-2495 0.157895 48.5 accD_p5 accD 5 ATGCTTGCAGGCTAATGTGT ACTTCCTCTTCTAGACCCCG 20 20 45.0 55.0 58.2 57.9 22.3 0.0 0.303552 28016 29494 2529-2534 0.157895 47.9 rpl20-rps12_p1 rpl20-rps12 1 CCACAAACGCCGAAAATCTC TGTCCCCAACAGAAGCAAAA 20 20 50.0 45.0 58.0 57.8 0.0 0.0 0.187755 36299 37044 1172-1189 0.157895 48.5 rpl20-rps12_p2 rpl20-rps12 2 CCACAAACGCCGAAAATCTC TTGTCCCCAACAGAAGCAAA 20 20 50.0 45.0 58.0 57.8 0.0 0.0 0.187755 36299 37044 1173-1190 0.157895 48.5 rpl20-rps12_p3 rpl20-rps12 3 CCACAAACGCCGAAAATCTC ATTTGTCAGCCGTATGCAGT 20 20 50.0 45.0 58.0 57.9 0.0 0.0 0.150113 36299 37044 1449-1466 0.157895 48.4 rpl20-rps12_p4 rpl20-rps12 4 AGACGAGTGATCCACAAACG ATTTGTCAGCCGTATGCAGT 20 20 50.0 45.0 57.9 57.9 0.0 0.0 0.179853 36299 37044 1460-1477 0.157895 48.1 rpl20-rps12_p5 rpl20-rps12 5 GCCCGTATTCGAGAAGCTAG ATTTGTCAGCCGTATGCAGT 20 20 55.0 45.0 57.9 57.9 0.0 0.0 0.167142 36299 37044 1893-1893 0.105263 42.9 psbB_p1 psbB 1 TGAAAGGGGGCTATCTCGAT CCGTCGCAACAACTAAGAGA 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.163479 39285 40811 2200-2251 0.368421 64.8 psbB_p2 psbB 2 ATTTTTGGTGAAAGGGGGCT CCGTCGCAACAACTAAGAGA 20 20 45.0 50.0 57.9 57.9 0.0 0.0 0.177195 39285 40811 2235-2259 0.263158 56.4 psbB_p3 psbB 3 TGAAAGGGGGCTATCTCGAT GTATTGTAGGTGCTTCGCGA 20 20 50.0 50.0 57.9 58.1 0.0 3.7 0.185881 39285 40811 2080-2112 0.157895 52.8 psbB_p4 psbB 4 TGAAAGGGGGCTATCTCGAT TGGTATTGTAGGTGCTTCGC 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.121695 39285 40811 2114-2114 0.105263 43.2 psbB_p5 psbB 5 ATTTTTGGTGAAAGGGGGCT TGGTATTGTAGGTGCTTCGC 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.135411 39285 40811 2122-2122 0.105263 43.1 petD-rps11_p1 petD-rps11 1 CAAAATCCATTTCGCCGTCC TGGAAGAGACGCAGCATTAC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.102080 44336 45350 668-1256 0.578947 74.3 petD-rps11_p2 petD-rps11 2 GGTTTCAATACCTGCGGGAT CACAATGGCTGTAGACCTCC 20 20 50.0 55.0 57.9 58.0 21.4 0.0 0.082621 44336 45350 647-1256 0.526316 72.4 petD-rps11_p3 petD-rps11 3 GGTTTCAATACCTGCGGGAT TGGAAGAGACGCAGCATTAC 20 20 50.0 50.0 57.9 58.0 21.4 0.0 0.062927 44336 45350 726-1314 0.421053 68.3 petD-rps11_p4 petD-rps11 4 TTCGCCGTCCAGTATCTACT CACAATGGCTGTAGACCTCC 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.078282 44336 45350 579-1188 0.368421 66.1 petD-rps11_p5 petD-rps11 5 TTCGCCGTCCAGTATCTACT TGGAAGAGACGCAGCATTAC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.058589 44336 45350 658-1246 0.315789 64.2 rpl16_p1 rpl16 1 AGCATATCGCCGATTGCTAG GGCGGAAGAAGCAGATACAA 20 20 50.0 50.0 58.0 58.0 24.8 0.0 0.025988 47248 48475 1587-1920 0.368421 66.5 rpl16_p2 rpl16 2 GCATATCGCCGATTGCTAGA GGCGGAAGAAGCAGATACAA 20 20 50.0 50.0 58.0 58.0 19.7 0.0 0.027884 47248 48475 1586-1919 0.368421 66.5 rpl16_p3 rpl16 3 GCTCTGACGACTTCCGATTT GGCGGAAGAAGCAGATACAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.026507 47248 48475 1803-2000 0.315789 64.4 rpl16_p4 rpl16 4 AGCATATCGCCGATTGCTAG TTGGCGGAAGAAGCAGATAC 20 20 50.0 50.0 58.0 58.0 24.8 0.0 0.025988 47248 48475 1725-1922 0.263158 62.3 rpl16_p5 rpl16 5 GCTCTGACGACTTCCGATTT TTGGCGGAAGAAGCAGATAC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.026507 47248 48475 1805-2002 0.263158 62.3 rpl2_p1 rpl2 1 GAACGACCCCTAATTCGGTC ATTGGCGAAGTCGTAGGTTC 20 20 55.0 50.0 58.1 58.0 8.5 2.3 0.074064 50077 50889 1546-1576 0.157895 48.1 rpl2_p2 rpl2 2 GAACGACCCCTAATTCGGTC GGCGAAGTCGTAGGTTCAAT 20 20 55.0 50.0 58.1 58.0 8.5 2.3 0.074064 50077 50889 1543-1573 0.157895 48.1 rpl2_p3 rpl2 3 GAACGACCCCTAATTCGGTC GAGACGCGGATCAAAGAGTT 20 20 55.0 50.0 58.1 58.0 8.5 12.5 0.070425 50077 50889 2032-2057 0.157895 47.9 rpl2_p4 rpl2 4 GAACGACCCCTAATTCGGTC GTTGAACCCGGTAAATTGCG 20 20 55.0 50.0 58.1 58.0 8.5 0.0 0.096302 50077 50889 2061-2086 0.157895 47.7 rpl2_p5 rpl2 5 GAACGACCCCTAATTCGGTC TTGCGTGAAAGTAGGAGACG 20 20 55.0 50.0 58.1 57.9 8.5 0.0 0.128420 50077 50889 2046-2071 0.157895 47.5