Hotspot

psaC-ndhE

SSC · 124000-124644 · 645 bp

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Pi 0.00958

MarkerSeek score 63.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 645
variable_sites: 48
pi: 0.00958
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGTCCCACGGTATCATTTCC	AAGCTGCTATTGGACTTGCT	55.0	45.0	58.4	57.5	778-919 bp	1.0	81.6
psaC-ndhE_p2	GGCAAGCTCGTACACATTGA	AAGCTGCTATTGGACTTGCT	50.0	45.0	58.6	57.5	745-886 bp	1.0	80.6
psaC-ndhE_p3	GTCCCACGGTATCATTTCCA	AAGCTGCTATTGGACTTGCT	50.0	45.0	57.3	57.5	777-918 bp	1.0	79.4
psaC-ndhE_p4	CGTCCCACGGTATCATTTCC	GAAGCTGCTATTGGACTTGC	55.0	50.0	58.4	57.2	779-920 bp	1.0	78.9
psaC-ndhE_p5	GCAAGCTCGTACACATTGAG	AAGCTGCTATTGGACTTGCT	50.0	45.0	57.2	57.5	744-885 bp	1.0	78.8