Hotspot

ndhC-trnV-UAC

LSC · 52691-54144 · 1454 bp

Back to Curcuma

Pi 0.00254
MarkerSeek score 55.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1454
variable_sites
34
pi
0.00254
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.96
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1530-1758 bp 1.0 83.9
ndhC-trnV-UAC_p2 CCTAACCGGGGCTAAAACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1531-1759 bp 1.0 83.8
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1524-1752 bp 1.0 83.0
ndhC-trnV-UAC_p4 CCTAACCGGGGCTAAAACTC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1613-1841 bp 1.0 81.6
ndhC-trnV-UAC_p5 CTAACCGGGGCTAAAACTCC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1612-1840 bp 1.0 81.6