Hotspot

trnE-UUC-trnT-GGU

LSC · 32396-33249 · 854 bp

Back to Curcuma

Pi 0.00584

MarkerSeek score 56.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 28
pi: 0.00584
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCCTTTCGTAGTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	969-1032 bp	1.0	83.8
trnE-UUC-trnT-GGU_p2	TCCTTTCGTAGTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	948-1011 bp	1.0	83.5
trnE-UUC-trnT-GGU_p3	TCCTTTCGTAGTACCCCCAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	945-1008 bp	1.0	83.3
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	933-996 bp	1.0	83.0
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	912-975 bp	1.0	83.0