Hotspot

trnD-GUC-trnY-GUA

LSC · 31750-32184 · 435 bp

Back to Curcuma

Pi 0.00417
MarkerSeek score 56.30
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
435
variable_sites
19
pi
0.00417
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGATCCTAGTTCGGGACTGA AATTCTTCCTGGGTCGATGC 55.0 50.0 57.9 58.0 613-634 bp 1.0 85.6
trnD-GUC-trnY-GUA_p2 GATCGGATCCTAGTTCGGGA AATTCTTCCTGGGTCGATGC 55.0 50.0 58.1 58.0 617-638 bp 1.0 85.4
trnD-GUC-trnY-GUA_p3 GGATCCTAGTTCGGGACTGA GGGGACGGACTGTAAATTCG 55.0 55.0 57.9 58.4 580-601 bp 1.0 83.4
trnD-GUC-trnY-GUA_p4 GGATCCTAGTTCGGGACTGA GGGACGGACTGTAAATTCGT 55.0 50.0 57.9 57.6 579-600 bp 1.0 83.2
trnD-GUC-trnY-GUA_p5 ATCGGATCCTAGTTCGGGAC AATTCTTCCTGGGTCGATGC 55.0 50.0 58.4 58.0 616-637 bp 1.0 83.1