Hotspot

trnC-GCA-petN

LSC · 28220-29048 · 829 bp

Back to Curcuma

Pi 0.00599

MarkerSeek score 47.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 31
pi: 0.00599
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATTTGGATCGGATTTGGCGA	AGTCCACTCCTTCCCCATAC	45.0	55.0	57.9	58.1	1003-1014 bp	1.0	81.8
trnC-GCA-petN_p2	ATTTGGATCGGATTTGGCGA	GTCCACTCCTTCCCCATACT	45.0	55.0	57.9	58.1	1002-1013 bp	1.0	81.8
trnC-GCA-petN_p3	ATTTGGATCGGATTTGGCGA	AAAGTCCACTCCTTCCCCAT	45.0	50.0	57.9	58.2	1005-1016 bp	1.0	80.9
trnC-GCA-petN_p4	ATTTGGATCGGATTTGGCGA	CCTCCCTAAAGTCCACTCCT	45.0	55.0	57.9	57.7	1012-1022 bp	0.904762	76.7
trnC-GCA-petN_p5	ATTTGGATCGGATTTGGCGA	TACCCCTCCCTAAAGTCCAC	45.0	55.0	57.9	57.7	1021-1026 bp	0.857143	73.2