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atpH-atpI

LSC · 13225-14664 · 1440 bp

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Pi 0.00388

MarkerSeek score 60.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1440
variable_sites: 38
pi: 0.00388
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.0	57.9	1496-1568 bp	1.0	85.1
atpH-atpI_p2	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.0	57.9	1579-1651 bp	1.0	84.9
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.9	57.9	1552-1624 bp	1.0	84.4
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.2	57.9	1497-1569 bp	1.0	83.9
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	ATTTTTGCAACCTTAGCCGC	55.0	45.0	58.2	57.9	1556-1628 bp	1.0	83.8