Hotspot

trnQ-UUG-psbK

LSC · 6396-6739 · 344 bp

Back to Curcuma

Pi 0.00855

MarkerSeek score 46.40

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 16
pi: 0.00855
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GAAGGATTCGAACCTCCGAG	CGGGCAATTTGGCAAAGAAA	55.0	45.0	57.8	58.1	486-515 bp	1.0	80.7
trnQ-UUG-psbK_p2	AGGATTCGAACCTCCGAGTA	CGGGCAATTTGGCAAAGAAA	50.0	45.0	57.6	58.1	484-513 bp	1.0	79.0
trnQ-UUG-psbK_p3	AAGGATTCGAACCTCCGAGT	CGGGCAATTTGGCAAAGAAA	50.0	45.0	58.4	58.1	485-514 bp	1.0	78.9
trnQ-UUG-psbK_p4	GAGTAACGGGACCAAAACCC	CGGGCAATTTGGCAAAGAAA	55.0	45.0	58.5	58.1	469-498 bp	1.0	78.8
trnQ-UUG-psbK_p5	ATTCGAACCTCCGAGTAACG	CGGGCAATTTGGCAAAGAAA	50.0	45.0	57.4	58.1	481-510 bp	1.0	78.0