Hotspot

rps16-trnQ-UUG

LSC · 5917-6322 · 406 bp

Back to Curcuma

Pi 0.0209
MarkerSeek score 61.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
406
variable_sites
27
pi
0.0209
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 480-1570 bp 1.0 86.6
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 478-1568 bp 1.0 86.6
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 486-1576 bp 1.0 86.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 462-1552 bp 1.0 85.9
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 484-1574 bp 1.0 85.5