Hotspot

ycf2

Genome · 39933-47468 · 7536 bp

Back to Cupressus

Pi 0.0067

MarkerSeek score 49.10

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 7536
variable_sites: 93
pi: 0.0067
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ACTTCCGTCTCTGAAAAGGG	GCAGAAGAAGAATTTGCTCGT	50.0	42.9	57.2	57.2	221-227 bp	1.0	68.0
ycf2_p2	ACTTCCGTCTCTGAAAAGGG	AGCAGAAGAAGAATTTGCTCG	50.0	42.9	57.2	56.9	222-228 bp	1.0	65.7
ycf2_p3	AACTTCCGTCTCTGAAAAGGG	GCAGAAGAAGAATTTGCTCGT	47.6	42.9	57.9	57.2	222-228 bp	1.0	65.7
ycf2_p4	CTTCCGTCTCTGAAAAGGGA	GCAGAAGAAGAATTTGCTCGT	50.0	42.9	56.9	57.2	220-226 bp	1.0	65.7
ycf2_p5	ACTTCCGTCTCTGAAAAGGG	GCAGAAGAAGAATTTGCTCGTT	50.0	40.9	57.2	57.8	221-227 bp	1.0	65.3