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psbM-petN

Genome · 90829-91431 · 603 bp

Back to Cupressus

Pi 0.0124

MarkerSeek score 60.60

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 603
variable_sites: 15
pi: 0.0124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GTCATTTACGCGCAAACGAA	TGGGCTGCTCTAATGGTAGT	45.0	50.0	58.1	58.1	714-738 bp	1.0	80.3
psbM-petN_p2	TCATTTACGCGCAAACGAAG	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.8	58.1	713-737 bp	1.0	79.0
psbM-petN_p3	GCAAACGAAGCCTCAAAGTG	TGGGCTGCTCTAATGGTAGT	50.0	50.0	58.2	58.1	703-727 bp	1.0	78.9
psbM-petN_p4	TATTCATTGCAGTGCCCACT	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.5	58.1	746-770 bp	1.0	76.6
psbM-petN_p5	ACGAAGCCTCAAAGTGAACT	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.4	58.1	699-723 bp	1.0	75.9