Hotspot

rps15-ndhH

Genome · 66674-66786 · 113 bp

Back to Cupressus

Pi 0.0147

MarkerSeek score 58.80

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: Genome
length_bp: 113
variable_sites: 3
pi: 0.0147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	AGATATTCGTGGACTGCGTT	TGATGGATGATGAGGACCCA	45.0	50.0	57.0	57.8	193-198 bp	1.0	74.8
rps15-ndhH_p2	AGATATTCGTGGACTGCGTT	GGTCTTTCTCCCTAGCAACC	45.0	55.0	57.0	57.6	158-163 bp	1.0	74.0
rps15-ndhH_p3	AGATATTCGTGGACTGCGTT	AAGTCGAAGAACACCATGCA	45.0	45.0	57.0	57.7	214-219 bp	1.0	73.9
rps15-ndhH_p4	AGATATTCGTGGACTGCGTT	AGTCGAAGAACACCATGCAT	45.0	45.0	57.0	57.5	213-218 bp	1.0	72.2
rps15-ndhH_p5	AGATATTCGTGGACTGCGTT	TGAGGTCTTTCTCCCTAGCA	45.0	50.0	57.0	57.4	161-166 bp	1.0	72.2