Hotspot

rrn4.5-rrn5

Genome · 60992-61309 · 318 bp

Back to Cupressus

Pi 0.011
MarkerSeek score 59.00
Primer available yes
Label rrn4.5-rrn5

Feature Metadata

label_name
rrn4.5-rrn5
feature_type
igs
region
Genome
length_bp
318
variable_sites
7
pi
0.011
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn4.5-rrn5_p1 CCACCTCAAGATGAGTGCTC GATTGGTGTGGTTCCCCTAC 55.0 55.0 58.0 57.9 471-476 bp 1.0 80.9
rrn4.5-rrn5_p2 CCACCTCAAGATGAGTGCTC AAGTTCGGGATGGATTGGTG 55.0 50.0 58.0 57.9 483-488 bp 1.0 80.8
rrn4.5-rrn5_p3 CCACCTCAAGATGAGTGCTC ACCAAGTTCGGGATGGATTG 55.0 50.0 58.0 57.9 486-491 bp 1.0 80.8
rrn4.5-rrn5_p4 CCACCTCAAGATGAGTGCTC CAAGTTCGGGATGGATTGGT 55.0 50.0 58.0 57.9 484-489 bp 1.0 80.8
rrn4.5-rrn5_p5 CCACCTCAAGATGAGTGCTC CACCAAGTTCGGGATGGATT 55.0 50.0 58.0 57.9 487-492 bp 1.0 80.8