Hotspot

trnA-UGC-rrn23

Genome · 57784-57926 · 143 bp

Back to Cupressus

Pi 0.00816

MarkerSeek score 60.40

Primer available yes

Label trnA-UGC-rrn23

Feature Metadata

label_name: trnA-UGC-rrn23
feature_type: igs
region: Genome
length_bp: 143
variable_sites: 2
pi: 0.00816
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-rrn23_p1	TAGACAGTGGTTGACCCTGT	GTCGATTAACACGCCCTTCT	50.0	50.0	57.9	58.0	262-274 bp	1.0	80.7
trnA-UGC-rrn23_p2	GGTTCGAGTCCGCTTATCTC	GTCGATTAACACGCCCTTCT	55.0	50.0	57.9	58.0	229-241 bp	1.0	80.7
trnA-UGC-rrn23_p3	GTTCGAGTCCGCTTATCTCC	GTCGATTAACACGCCCTTCT	55.0	50.0	57.9	58.0	228-240 bp	1.0	80.7
trnA-UGC-rrn23_p4	TAGACAGTGGTTGACCCTGT	ATTCGGGAATCTCCGGATCT	50.0	50.0	57.9	58.0	312-324 bp	1.0	80.4
trnA-UGC-rrn23_p5	GTTCGAGTCCGCTTATCTCC	ATTCGGGAATCTCCGGATCT	55.0	50.0	57.9	58.0	278-290 bp	1.0	80.3