Hotspot

trnV-GAC-rrn16

Genome · 52843-53575 · 733 bp

Back to Cupressus

Pi 0.0152
MarkerSeek score 71.40
Primer available yes
Label trnV-GAC-rrn16

Feature Metadata

label_name
trnV-GAC-rrn16
feature_type
igs
region
Genome
length_bp
733
variable_sites
21
pi
0.0152
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rrn16_p1 ACCTTGACGTGGTGAAAGTC ACTTCCCGTTTGACTTGCAT 50.0 45.0 58.1 58.0 850-891 bp 1.0 84.2
trnV-GAC-rrn16_p2 CCTTGACGTGGTGAAAGTCA ACTTCCCGTTTGACTTGCAT 50.0 45.0 58.1 58.0 849-890 bp 1.0 84.2
trnV-GAC-rrn16_p3 ACCTTGACGTGGTGAAAGTC GGGCAGGTTCTTACGCATTA 50.0 50.0 58.1 58.0 896-937 bp 1.0 83.9
trnV-GAC-rrn16_p4 CCTTGACGTGGTGAAAGTCA GGGCAGGTTCTTACGCATTA 50.0 50.0 58.1 58.0 895-936 bp 1.0 83.9
trnV-GAC-rrn16_p5 ACCTTGACGTGGTGAAAGTC AAACACCACTTCCCGTTTGA 50.0 45.0 58.1 57.9 857-898 bp 1.0 83.3