Hotspot

ycf1-trnL-CAA

Genome · 38751-39634 · 884 bp

Back to Cupressus

Pi 0.0196

MarkerSeek score 60.80

Primer available yes

Label ycf1-trnL-CAA

Feature Metadata

label_name: ycf1-trnL-CAA
feature_type: igs
region: Genome
length_bp: 884
variable_sites: 30
pi: 0.0196
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnL-CAA_p1	CAAGTTCTTCTCGAGGACCG	CATTTTGAGTGTCGCGTGTC	55.0	50.0	58.0	58.1	1639-1767 bp	1.0	85.7
ycf1-trnL-CAA_p2	CAAGTTCTTCTCGAGGACCG	GACATTTTGAGTGTCGCGTG	55.0	50.0	58.0	58.1	1641-1769 bp	1.0	85.7
ycf1-trnL-CAA_p3	TGGATGAGAAGACCACCAGT	CATTTTGAGTGTCGCGTGTC	50.0	50.0	58.0	58.1	1141-1269 bp	1.0	85.6
ycf1-trnL-CAA_p4	TTTGAAGCCAAGGGTGTCTC	CATTTTGAGTGTCGCGTGTC	50.0	50.0	58.0	58.1	1264-1384 bp	0.75	74.4
ycf1-trnL-CAA_p5	TTTGAAGCCAAGGGTGTCTC	GACATTTTGAGTGTCGCGTG	50.0	50.0	58.0	58.1	1266-1386 bp	0.75	74.4